Estrous Stage- and Animal Age-Independent Superovulation in the BrlHan:WIST@Jcl(GALAS) Rat

• SOTOMARU Yusuke

  Central Institute for Experimental Animals

• KAMISAKO Tsutomu

  JAC Inc.

• HIOKI Kyoji

  Central Institute for Experimental Animals

抄録

In most strains of rats, the effects of treatment for the induction of superovulation show major strain differences and are strongly influenced by the stage of the estrous cycle. This study demonstrated, however, that superovulation was easily induced in Wistar strain Brl Han:WIST@Jcl(GALAS) rats by PMSG and hCG administration. To confirm the effects of such treatment, we studied age differences in egg collection efficiency. After superovulation was induced by intraperitoneal administration of 150 IU/kg PMSG and 75 IU/kg hCG given 48 h apart, the mean numbers of oocytes obtained from rats at 4, 8, 12, 20 and 28 weeks of age were 38.9, 33.5, 46.1, 26.9 and 21.3, respectively. No differences caused by the estrous stage at the PMSG administration were observed. In an embryo transfer experiment, fertilized eggs obtained from superovulated rats at each week of age showed equivalent viability until full-term to those from untreated rats. These results suggest that estrous stage-independent superovulation is effective not only in the pre-pubertal stage but also in adult rats.<br>

収録刊行物

• Experimental Animals

  Experimental Animals 54 (2), 137-141, 2005

  公益社団法人 日本実験動物学会

キーワード

• estrous cycle
• hCG
• PMSG
• superovulation

詳細情報

• CRID: 1390282680023775488
• NII論文ID: 10015525802
• NII書誌ID: AA11032321
• DOI: 10.1538/expanim.54.137
• COI: 1:CAS:528:DC%2BD2MXlsFSqt7c%3D
• ISSN: 18817122
• ISSN: 00075124
• ISSN: 13411357
• NDL書誌ID: 7304391
• PubMed: 15897622
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7304391
• Web Site: http://www.jstage.jst.go.jp/article/expanim/54/2/54_2_137/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2005220223
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可