Effects of a phenolic compound, resveratrol, on the renal function and costimulatory adhesion molecule CD86 expression in rat kidneys with ischemia/reperfusion injury

• Saito Mitsuru

  Departments of Urology, Akita University School of Medicine

• Satoh Shigeru

  Departments of Urology, Akita University School of Medicine

• Kojima Naosuke

  Departments of Anatomy, Akita University School of Medicine

• Tada Hitoshi

  Departments of Pharmaceutical Science, Akita University School of Medicine

• Sato Mitsuru

  Departments of Anatomy, Akita University School of Medicine

• Suzuki Toshio

  Departments of Pharmaceutical Science, Akita University School of Medicine

• Senoo Haruki

  Departments of Anatomy, Akita University School of Medicine

• Habuchi Tomonori

  Departments of Urology, Akita University School of Medicine

抄録

Recent studies have suggested that an ischemia/reperfusion (I/R) injury enhances the expression of costimulatory adhesion molecules on the vascular endothelium. In the present study, we investigated the protective effects of resveratrol, a phenolic product, on the renal function and expression of CD86 in rat kidneys with I/R injury. Wistar rats were divided into four groups;1) an I/R group with right nephrectomy and 1-hour clamping of the left renal pedicle; 2) a vehicle group, I/R plus 10% ethanol (0.1ml/kg/day) administered by intra-peritoneal injection from day —1 through to 7; 3) a resveratrol group, I/R plus 4mg/kg/day of resveratrol; and 4) a sham group. Blood samples were obtained via the tail vein at 1 day before, and 1, 3, and 7 days after the operation (day 0) for the measurement of serum creatinine (Scr) levels. The expression of CD86 protein was analyzed by immunofluorescence staining, and the level of CD86 messenger RNA (mRNA) was evaluated quantitatively by a real-time reverse transcription-polymerase chain reaction (RT-PCR) in the renal cortex at day 3. Scr levels of the resveratrol group were significantly lower than those of the I/R and vehicle groups on days 1 and 3 after the operation. From the immunohistochemical study, the expression of CD86 in the glomerular endothelium and peritubular vessels was found to be attenuated in the resveratrol group compared with the I/R or vehicle group. In the resveratrol group, the CD86 mRNA level was significantly lower than that in the I/R or vehicle group, and it was significantly decreased by about one fifth of that in the sham group. Our results suggest that resveratrol markedly reduces renal dysfunction and attenuates the mRNA and protein expression of CD86 following I/R injury.

収録刊行物

• Ａｒｃｈｉｖｅｓ　ｏｆ　Ｈｉｓｔｏｌｏｇｙ　ａｎｄ　Ｃｙｔｏｌｏｇｙ

  Ａｒｃｈｉｖｅｓ　ｏｆ　Ｈｉｓｔｏｌｏｇｙ　ａｎｄ　Ｃｙｔｏｌｏｇｙ 68 (1), 41-49, 2005

  国際組織細胞学会

キーワード

• Histology

詳細情報

• CRID: 1390001206412464384
• NII論文ID: 130004462636
• NII論文ID: 10015564943
• NII書誌ID: AA1068990X
• DOI: 10.1679/aohc.68.41
• ISSN: 13491717
• ISSN: 09149465
• PubMed: 15827377
• Web Site: http://www.jstage.jst.go.jp/article/aohc/68/1/68_1_41/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2005247008
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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