Suplatast Tosilate Inhibits Human Mast Cell Development from Cord Blood Progenitors

• Fujisawa Akihiro

  Departments of Dermatology, Kyoto University Graduate School of Medicine

• Kambe Naotomo

  Departments of Dermatology, Kyoto University Graduate School of Medicine

• Nakahata Tatsutoshi

  Pediatrics, Kyoto University Graduate School of Medicine

• Matsuda Hiroshi

  Laboratory of Molecular Pathology and Therapeutics, Division of Animal Life Science, Graduate School, Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology

• Miyachi Yoshiki

  Departments of Dermatology, Kyoto University Graduate School of Medicine

抄録

Background: Localized mast cell accumulation has been reported in a number of human diseases. Suplatast tosilate has been reported to inhibit IL-3-dependent murine mast cell development, and therefore is expected to suppress mast cell growth proliferation at disease sites. However, there are differences between the growth factors required by human and mouse mast cells.<br> Methods: Human mast cells were grown in serum-free culture condition from cord blood mononuclear cells with suplatast tosilate. Mast cell number was assessed from the cell numbers counted by a flow cytometer with constant flow rate and Kit+ percentage. Immunochemistry for tryptase was also examined on the cytocentrifuged preparations.<br> Results: Suplatast tosilate (100 μg/ml ) dramatically inhibited human mast cell development from cord blood progenitors. On the other hand, the growth of 8-wk old mast cells that developed in serum-free media, and which reached over 95% in purity and still maintained proliferation ability, and the growth of the human mast cell line HMC-1 cells were not inhibited by suplatast tosilate.<br> Conclusions: Suplatast tosilate may be useful for control of human mast cell numbers at the local sites by inhibiting their development from their progenitors.<br>

収録刊行物

• Allergology International

  Allergology International 54 (2), 311-315, 2005

  一般社団法人日本アレルギー学会

キーワード

• cord blood progenitors
• human mast cells
• stem cell factor
• suplatast tosilate

詳細情報

• CRID: 1390282679609975296
• NII論文ID: 10015655875
• NII書誌ID: AA11091750
• DOI: 10.2332/allergolint.54.311
• ISSN: 14401592
• ISSN: 13238930
• Web Site: http://api.elsevier.com/content/article/PII:S132389301531039X?httpAccept=text/xml
• Web Site: http://api.elsevier.com/content/article/PII:S132389301531039X?httpAccept=text/plain
• Web Site: https://search.jamas.or.jp/link/ui/2006262255
• 本文言語コード: en
• データソース種別:
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  • Crossref
  • CiNii Articles
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