凍結培養細胞を用いた培養複合口腔粘膜作製に関する基礎的研究

• 小山 貴寛

  新潟大学大学院医歯学総合研究科口腔生命科学専攻口腔健康科学講座顎顔面口腔外科学分野

• 飯田 明彦

  新潟大学大学院医歯学総合研究科口腔生命科学専攻口腔健康科学講座顎顔面口腔外科学分野

• 芳澤 享子

  新潟大学大学院医歯学総合研究科口腔生命科学専攻顎顔面再建学講座組織再建口腔外科学分野

• 齊藤 力

  新潟大学大学院医歯学総合研究科口腔生命科学専攻顎顔面再建学講座組織再建口腔外科学分野

• 高木 律男

  新潟大学大学院医歯学総合研究科口腔生命科学専攻口腔健康科学講座顎顔面口腔外科学分野

書誌事項

タイトル別名

• Basic study of an ex vivo produced oral mucosa equivalent by cryopreserved oral keratinocytes
• トウケツ バイヨウ サイボウ オ モチイタ バイヨウ フクゴウ コウクウ ネンマク サクセイ ニ カンスル キソテキ ケンキュウ

抄録

The main objective of this study was to fabricate an ex vivo produced oral mucosa equivalent (EVPOME) using cryopreserved autogenous keratinocytes and to assess their suitability as graft materials for oral mucosa the same as EVPOME using non-cryopreserved autogenous keratinocytes.<BR>Human oral keratinocytes of passage 2 were harvested and frozen at -80°C or -196°C with 10% DMSO, and cryopreserved for 3 or 6 months respectively. Cells that were not cryopreserved were used as a control. The cell growth curve of each experimental group was reconstructed and the population-doubling time (PDT) was calculated to evaluate cell proliferation ability. Cells of each group were also seeded onto AlloDerm^® to fabricate equivalents. Histology of equivalents and their expression of immunoreactive proliferating cell nuclear antigen (PCNA), a proliferation marker, and filaggrin, a cell differentiation marker, were examined at 4, 11, and 18 days after seeding cells onto AlloDerm^®.<BR>Cell growth curves of each group were almost the same. PDT of the cells cryopreserved at -80°C for 6 months was longer than that of non-cryopreserved cells. Stratified epithelium on AlloDerm^® was observed on day 11 and 18 equivalents obtained using cryopreserved cells, the same as those using non-cryopreserved cells. Immunoreactivity for both PCNA and filaggrin was present in the epithelial cells of equivalents using both cryopreserved cells and non-cryopreserved cells.<BR>From these results, it appears that EVPOME using cryopreserved cells is applicable for clinical use in view of their proliferative and differentiated keratinized state as well as EVPOME using non-cryopreserved cells.

収録刊行物

• 日本口腔科学会雑誌

  日本口腔科学会雑誌 54 (2), 253-263, 2005

  特定非営利活動法人 日本口腔科学会

キーワード

• 組織工学
• 口腔粘膜上皮細胞
• 凍結保存

詳細情報

• CRID: 1390001206436240000
• NII論文ID: 130004114510
• NII論文ID: 10015656677
• NII書誌ID: AN00189050
• DOI: 10.11277/stomatology1952.54.253
• ISSN: 21850461
• ISSN: 00290297
• NDL書誌ID: 7675348
• Web Site: http://id.ndl.go.jp/bib/7675348
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7675348
• Web Site: https://search.jamas.or.jp/link/ui/2005189602
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可