Determination of Peroxidase Encapsulated in Liposomes Using Homogentisic Acid .GAMMA.-Lactone Chemiluminescence

• KAMIDATE Tamio

  Graduate School of Engineering, Hokkaido University

• KIKUCHI Nobuhito

  Graduate School of Engineering, Hokkaido University

• ISHIDA Akihiko

  Graduate School of Engineering, Hokkaido University

• TANI Hirofumi

  Graduate School of Engineering, Hokkaido University

Bibliographic Information

Other Title

• Determination of Peroxidase Encapsulated in Liposomes Using Homogentisic Acid γ-Lactone Chemiluminescence
• Determination of Peroxidase Encapsulated in Liposomes Using Homogentisic Acid ガンマ Lactone Chemiluminescence

Abstract

Homogentisic acid γ-lactone (HAL) chemiluminescence (CL) was applied to the determination of horseradish peroxidase (HRP) encapsulated in liposomes. HRP was detected after the lysis of HRP-trapped liposomes with Triton X-100. CL response rate, detection limit and linear range of calibration curve for HRP in HAL CL were compared with those in p-iodophenol (p-IP)-enhanced luminol CL. Maximal light emission in HAL CL appeared more rapidly compared to that in p-IP enhanced luminol CL, thus resulting in remarkable reduction of CL measurement time. The detection limit for HRP in HAL CL was the same as that in p-IP-enhanced luminol CL. The linear range of calibration curve for HRP in HAL CL was improved by a factor of 50 compared with that in p-IP-enhanced luminol CL. From these results, it was found that HAL CL were superior to p-IP-enhanced luminol CL for the determination of HRP encapsulated in liposomes.

Journal

• Analytical Sciences

  Analytical Sciences 21 (6), 701-704, 2005

  The Japan Society for Analytical Chemistry

Keywords

• Homogentisic acid γ-lactone (HAL)
• chemiluminescence (CL)
• determination of horseradish peroxidase (HRP)

Details

• CRID: 1390001204257904768
• NII Article ID: 10016142010
• NII Article ID: 120006531118
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.21.701
• COI: 1:CAS:528:DC%2BD2MXlsFersLs%3D
• ISSN: 13482246
• ISSN: 09106340
• HANDLE: 2115/71701
• NDL BIB ID: 7332778
• PubMed: 15984210
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7332778
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.21.701.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.21.701/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/21/6/21_6_701/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed