Cytotoxic and Membrane Perturbation Effects of a Novel Amyloid Forming Model Peptide Poly (Leucine-Glutamic Acid)
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- Jayakumar Rajiadas
- Bio-organic and Neurochemistry Division, Central Leather Research Institute
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- Murali Jayaraman
- Bio-organic and Neurochemistry Division, Central Leather Research Institute
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- Koteeswari Deenadayalan
- Bio-organic and Neurochemistry Division, Central Leather Research Institute
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- Gomathi Kannayiram
- Bio-organic and Neurochemistry Division, Central Leather Research Institute
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In the present study we have elucidated the toxicity of a novel amyloid forming model peptide, Poly (leucine-glutamic acid). The toxicity of the fibrils prepared from this peptide was analyzed in peripheral blood lymphocytes (PBL). The MTT reduction assay revealed that the viability of PBL decreases significantly upon treatment with Poly (leucine-glutamic acid) (Poly [LE]). Enhanced DCFH-DA fluorescence in treated cells suggests that peptide toxicity is probably mediated by the formation of free radicals. In vivo and in vitro biochemical studies indicated that Poly [LE] inactivates the antioxidant system of cells. Perturbation of Poly [LE] in a membrane lipid environment was assessed by circular dichroism (CD) using phosphotidyl choline-cholesterol bilayers. The CD results revealed that LE enhances its beta sheet content in a bilayer environment. Sequestration of Poly [LE] in lipid rafts demonstrates that it has a binding cleft similar to Aβ in lymphocyte raft domains. Nuclear membrane binding studies showed that Poly [LE] binds to nuclear membranes and may cause genotoxicity.
収録刊行物
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- The Journal of Biochemistry
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The Journal of Biochemistry 136 (4), 457-462, 2004
社団法人 日本生化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204964717184
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- NII論文ID
- 130003534743
- 10016202722
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- NII書誌ID
- AA00694073
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- COI
- 1:CAS:528:DC%2BD2MXit1Skur0%3D
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- ISSN
- 17562651
- 0021924X
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- NDL書誌ID
- 7212914
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- PubMed
- 15625314
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可