LexA Analog (dra0074) Is a Regulatory Protein That Is Irrelevant to recA Induction

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  • Sheng Duohong
    Institute of Nuclear-Agricultural Sciences, Zhejiang University
  • Zheng Zhiguo
    Institute of Nuclear-Agricultural Sciences, Zhejiang University
  • Tian Bing
    Institute of Nuclear-Agricultural Sciences, Zhejiang University
  • Shen Binghui
    Department of Radiation Biology, City of Hope National Medical Center and Beckman Research Institute
  • Hua Yuejin
    Institute of Nuclear-Agricultural Sciences, Zhejiang University

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  • LexA Analog (dra0074) Is a Regulatory Protein That Is Irrelevant to <i>recA</i> Induction

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The protein DRA0074 is suggested to be another LexA in Deinococcus radiodurans, having similar motifs and RecA-mediated cleavage activity to D. radiodurans LexA (dra0344). However, its function has not been studied. We disrupted the gene dra0074 and measured its effect on RecA induction using fusion translation, immunoblot, and proteomic analysis. Results showed that the product of gene dra0074 is not involved in RecA induction, but is a regulator of other metabolisms in D. radiodurans.

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