Simultaneous Determination of Single Nucleotide Polymorphisms of MDR1 Genes by Electrochemical DNA Chip

• NAKAMURA Tsutomu

  Department of Hospital Pharmacy, School of Medicine, Kobe University

• SAKAEDA Toshiyuki

  Department of Hospital Pharmacy, School of Medicine, Kobe University

• TAKAHASHI Masayoshi

  Corporate Research & Development Center, Toshiba Co.

• HASHIMOTO Koji

  Corporate Research & Development Center, Toshiba Co.

• GEMMA Nobuhiro

  Corporate Research & Development Center, Toshiba Co.

• MORIYA Yuka

  Department of Hospital Pharmacy, School of Medicine, Kobe University

• KOMOTO Chiho

  Department of Hospital Pharmacy, School of Medicine, Kobe University

• NISHIGUCHI Kohshi

  Department of Hospital Pharmacy, School of Medicine, Kobe University

• OKAMURA Noboru

  Department of Clinical Evaluation of Pharmacotherapy, Kobe University Graduate School of Medicine

• OKUMURA Katsuhiko

  Department of Hospital Pharmacy, School of Medicine, Kobe University Department of Clinical Evaluation of Pharmacotherapy, Kobe University Graduate School of Medicine

Abstract

  The on-chip genotyping system (“the electrochemical DNA chip”) has been developed as a more cost-effective genotyping system and was applied to MDR1 genotyping in the present study, which is required for wide use in clinical application and for personalized medication based on genotype. The electrochemical DNA chip was optimized and applied to simultaneous genotyping of four MDR1 polymorphisms (T-129C, C1236T, G2677(A,T) and C3435T) using synthetic model oligonucleotide DNA and human genomic DNA. The electrochemical DNA chip successfully gave the T-129C, C1236T, G2677(A,T) and C3435T genotypes, which were completely consistent with those determined by direct sequencing. In conclusion, the electrochemical DNA chip is useful for simultaneous determination of some genotypes and haplotypes, and efficient genotyping using this system can support future genotype-phenotype studies at a large scale.<br>

Journal

• Drug Metabolism and Pharmacokinetics

  Drug Metabolism and Pharmacokinetics 20 (3), 219-225, 2005

  The Japanese Society for the Study of Xenobiotics

Keywords

• DNA chip
• genotype
• <i>MDR1</i>

Details

• CRID: 1390001205179348096
• NII Article ID: 10016453944
• NII Book ID: AA1162652X
• DOI: 10.2133/dmpk.20.219
• ISSN: 18800920
• ISSN: 13474367
• PubMed: 15988125
• Web Site: http://api.elsevier.com/content/article/PII:S1347436715307552?httpAccept=text/xml
• Web Site: http://api.elsevier.com/content/article/PII:S1347436715307552?httpAccept=text/plain
• Web Site: https://search.jamas.or.jp/link/ui/2006262299
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed