Establishment of a SPF Colony on Human Apo(a) Transgenic Rabbits by Frozen-Thawed Embryo Transfer
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- WATANABE Nobuhiko
- Biotek Co. Ltd. Analytical Research Center for Experimental Sciences, Saga University
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- NAKAGAWA Hiroshi
- Biotek Co. Ltd.
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- KITAJIMA Shuji
- Analytical Research Center for Experimental Sciences, Saga University
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- LIU Enqi
- Analytical Research Center for Experimental Sciences, Saga University
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- MORIMOTO Masatoshi
- Analytical Research Center for Experimental Sciences, Saga University
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- WATANABE Teruo
- Saga University
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- FAN Jianglin
- Institute of Basic Medical Science, University of Tsukuba
Bibliographic Information
- Other Title
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- Establishment of a SPF Colony of Human Apo(a) Transgenic Rabbits by Frozen-Thawed Embryo Transfer
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Abstract
In the present study, embryo transfer was performed using frozen-thawed embryos to establish a SPF colony of human apolipoprotein (a) (apo(a)) transgenic rabbits. Apo(a) transgenic rabbits were kept under conventional condition and were infected with Bordetella bronchiseptica. Embryos at the morula stage were collected and stored in liquid nitrogen. After thawing, the in vitro survival rate was 84.6%, and 125 morphologically normal embryos were transferred to 6 SPF recipient rabbits. Four rabbits became pregnant and 23 live pups were born. PCR and Western blot analyses revealed that 9 of 23 pups were transgenic and expressed apo(a) protein. Microbiological tests showed all rabbits were free from infections. We succeeded in establishing a SPF colony of apo(a) transgenic rabbits. These rabbits are now maintained under a barrier system and are available for medical research.<br>
Journal
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- Experimental Animals
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Experimental Animals 54 (4), 353-357, 2005
Japanese Association for Laboratory Animal Science
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Keywords
Details 詳細情報について
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- CRID
- 1390001205046404480
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- NII Article ID
- 10016627654
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- NII Book ID
- AA11032321
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- COI
- 1:CAS:528:DC%2BD2MXpvFOju7w%3D
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- ISSN
- 18817122
- 00075124
- 13411357
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- NDL BIB ID
- 7363525
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- PubMed
- 16093649
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed