Evaluation of an ODS Column Modified with Zwitterionic/Nonionic Mixed Surfactants and Its Application to Direct Injection Determination of Inorganic Anions

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  • HASEGAWA Takuya
    Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
  • UMEMURA Tomonari
    Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
  • KOIDE Akira
    Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
  • CHIBA Koichi
    Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
  • UEKI Yuji
    Department of Chemistry, Faculty of Engineering, Gunma University
  • TSUNODA Kin-ichi
    Department of Chemistry, Faculty of Engineering, Gunma University
  • HARAGUCHI Hiroki
    Department of Applied Chemistry, Graduate School of Engineering, Nagoya University

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Abstract

An octadecylsilica (ODS) column modified with zwitterionic/nonionic mixed surfactants was evaluated for the direct injection determination of inorganic anions in biological fluids by ion chromatography. A zwitterionic surfactant (sulfobetaine-type) and a nonionic surfactant (polyoxyethylene-type) were used for a stationary-phase modification. When aqueous electrolyte solutions with concentrations of sub-mM to several mM were used as a mobile phase, the zwitterionic surfactant coated on the ODS surface exhibited unique separation selectivity for ionic species, while the nonionic surfactant coated on the ODS might have formed a hydrophilic network over the ODS surface and restricted matrix proteins from adsorbing on the stationary phase. Consequently, the mixed surfactant-modified column system allowed an efficient ion chromatographic separation of inorganic anions as well as a size-exclusive removal of column-fouling proteins. This separation system was applied to the direct injection determination of UV-absorbing anions in human saliva. The detection limits for nitrite, nitrate, iodide and thiocyanate were 3.1, 2.7, 4.5 and 25 µM, respectively, with UV detection at 210 nm (injection volume; 20 µl), and their relative standard deviations for 5 replicate measurements of saliva samples spiked with 100 µM each of those anions were 1.4, 0.9, 2.2 and 5.5%, respectively.

Journal

  • Analytical Sciences

    Analytical Sciences 21 (8), 913-916, 2005

    The Japan Society for Analytical Chemistry

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