In Vitro Inhibitory Effects of Cannabinoids on Progesterone 17.ALPHA.-Hydroxylase Activity in Rat Testis Microsomes

• Funahashi Tatsuya

  Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University

• Ikeuchi Hideharu

  Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University

• Yamaori Satoshi

  Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University

• Kimura Toshiyuki

  Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University

• Yamamoto Ikuo

  Department of Hygienic Chemistry, School of Pharmaceutical Sciences, Kyushu University of Health and Welfare

• Watanabe Kazuhito

  Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University

Bibliographic Information

Other Title

• In Vitro Inhibitory Effects of Cannabinoids on Progesterone 17α-Hydroxylase Activity in Rat Testis Microsomes
• In Vitro Inhibitory Effects of Cannabinoids on Progesterone 17 アルファ Hydroxylase Activity in Rat Testis Microsomes

Abstract

The inhibitory effects of three major cannabinoids [Δ⁹-tetrahydrocannabinol (THC), cannabidiol (CBD), cannabinol (CBN)] contained in marijuana, an abused drug, on progesterone 17α-hydroxylase activity in rat testis microsomes were investigated. Microsomal progesterone 17α-hydroxylase activity was significantly inhibited in the presence of more than 50 μM of Δ⁹-THC and CBN compared with control activity, and the IC₅₀ values for Δ⁹-THC and CBN were estimated to be 42.8 and 32.9 μM, respectively. CBD showed less but significant inhibitory effects on 17α-hydroxylase activity at concentrations greater than 100 μM, and the IC₅₀ value for the cannabinoid was estimated to be 290.9 μM. Kinetic analysis using double reciprocal plots showed that the type of inhibition by CBN was competitive, whereas that of Δ⁹-THC and CBD was the mixed type. These results suggest that the inhibition may be due to metabolic interactions between each cannabinoid and 17α-hydroxylase. <br>

Journal

• Journal of Health Science

  Journal of Health Science 51 (3), 369-375, 2005

  The Pharmaceutical Society of Japan

Keywords

• cannabinoid
• 17<i>α</i>-hydroxylase
• testis
• enzyme inhibition

Details

• CRID: 1390282679474715776
• NII Article ID: 10016657733
• NII Book ID: AA11316464
• DOI: 10.1248/jhs.51.369
• ISSN: 13475207
• ISSN: 13449702
• NDL BIB ID: 7318517
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7318517
• Web Site: http://www.jstage.jst.go.jp/article/jhs/51/3/51_3_369/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2006233813
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed