A New Paradigm of Protein Structures by Variable Pressure NMR

• KITAHARA Ryo

  RIKEN Spring-8 Center, RIKEN Harima Institute

• YOKOYAMA Shigeyuki

  RIKEN Genomic Sciences Center Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo

• AKASAKA Kazuyuki

  RIKEN Spring-8 Center, RIKEN Harima Institute Department of Biotechnological Science School of Biology -Oriented Science and Technology, Kinki University

Bibliographic Information

Other Title

• 可変圧力ＮＭＲ法による新しい蛋白質構造パラダイムの構築
• カヘン アツリョク NMRホウ ニ ヨル アタラシイ タンパクシツ コウゾウ パラダイム ノ コウチク

Abstract

A protein in solution is a thermodynamic entity existing in equilibria among multiple conformations from the folded to the unfolded. Various experimental results indicate that transitions to higher energy conformers are crucial for function in many proteins. However, our knowledge on higher energy conformers is extremely limited at present, compared to the knowledge about the folded counterparts. A newly emerging technique, the variable pressure NMR method, enables us to explore a much wider conformational space of proteins than hitherto explored by X-ray crystallography and conventional NMR technique, giving a new dynamic view of proteins that amply fluctuate over the entire conformational space.

Journal

• Journal of the Spectroscopical Society of Japan

  Journal of the Spectroscopical Society of Japan 55 (1), 10-20, 2006

  The Spectroscopical Society of Japan

Details

• CRID: 1390282681236841984
• NII Article ID: 10016735137
• NII Book ID: AN00222531
• DOI: 10.5111/bunkou.55.10
• COI: 1:CAS:528:DC%2BD28XislOntbY%3D
• ISSN: 18846785
• ISSN: 00387002
• ISSN: http://id.crossref.org/issn/00387002
• NDL BIB ID: 7848668
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7848668
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed