Characterization of Bombyx mori Sericins by the Partial Amino Acid Sequences

• Takasu Yoko

  National Institute of Agrobiological Sciences

• Yamada Hiromi

  National Institute of Agrobiological Sciences Center for Ecological Research, Kyoto University

• Saito Hitoshi

  National Institute of Agrobiological Sciences Present address: Department of Applied Biology, Kyoto Institute of Technology

• Tsubouchi Kozo

抄録

To confirm the correspondence between sericin proteins and sericin genes of Bombyx mori, the partial amino acid sequences of cocoon sericins were determined by N-terminal sequencing of lysyl endopeptidase fragments and were then compared to the polypeptides derived from the Ser1 gene. It was determined that sericin M, which is the abundant component of sericin, was a product of the Ser1 gene, whereas sericin A, which distributes mainly in the floss and the outer layer of the cocoon, was not. In addition, sericin P was considered to be a smaller product of the Ser1 gene based on the cleavage pattern in the enzymatic digestion. It is expected that the properties of sericin A are different from those of sericins M and P because it does not have 38-amino acid repeats that constitute the major portion of the Ser1 proteins.<br>

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｉｎｓｅｃｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ　ａｎｄ　Ｓｅｒｉｃｏｌｏｇｙ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｉｎｓｅｃｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ　ａｎｄ　Ｓｅｒｉｃｏｌｏｇｙ 74 (3), 103-109, 2005

  社団法人 日本蚕糸学会

キーワード

• <i>Bombyx mori</i>
• sericin
• amino acid sequence
• lysyl endopeptidase

詳細情報

• CRID: 1390282680172431744
• NII論文ID: 130004464099
• NII論文ID: 10016770298
• NII書誌ID: AA11558849
• DOI: 10.11416/jibs.74.103
• ISSN: 18847978
• ISSN: 13468073
• NDL書誌ID: 7692590
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7692590
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可