Probing Actin Polymerization-Driven Cell Motility Machinery by Single-Molecule Imaging

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  • WATANABE Naoki
    Department of Pharmacology, Graduate School of Medicine, Kyoto University PRESTO-JST
  • HIGASHIDA Chiharu
    Department of Pharmacology, Graduate School of Medicine, Kyoto University
  • MIYOSHI akushi
    Department of Pharmacology, Graduate School of Medicine, Kyoto University

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Other Title
  • アクチン重合が駆動する細胞運動の謎に挑む単分子イメージング
  • アクチン ジュウゴウ ガ クドウ スル サイボウ ウンドウ ノ ナゾ ニ イドム タンブンシ イメージング

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Abstract

Actin polymerization generates driving force for cell edge protrusion. Single-molecule speckle microscopy is a potent tool for probing association-dissociation kinetics of actin regulators with actin in living cells. The previous study of actin speckle analysis revealed the ceaseless actin filament turnover throughout lamellipodia of cultured fibroblasts. In order to understand how this fast actin filament lifetime is regulated, we are now extending application of single-molecule imaging to major actin end binding proteins. We also describe our discovery of processive actin capping by Formin-homology proteins, and discuss recent progress made on the mechanistic property and the structural feature of Formins’ interaction with the actin filament.<br>

Journal

  • Seibutsu Butsuri

    Seibutsu Butsuri 45 (6), 292-296, 2005

    The Biophysical Society of Japan General Incorporated Association

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