Tissue Inhibitors of Metalloproteinases (TIMP-1 and TIMP-2) Stimulate Osteoclast Differentiation

• Sobue Takanori

  Departments of Periodontology, Aichi-Gakuin University, School of Dentistry

• Oguchi Masayo

  Departments of Periodontology, Aichi-Gakuin University, School of Dentistry

• Tanaka Shigehisa

  Departments of Periodontology, Aichi-Gakuin University, School of Dentistry

• Koide Masanori

  Departments of Periodontology, Aichi-Gakuin University, School of Dentistry

• Ishihara Yuichi

  Departments of Periodontology, Aichi-Gakuin University, School of Dentistry

• Yamashita Kyoko

  Biochemistry, Aichi-Gakuin University, School of Dentistry

• Hayakawa Hirohisa

  Departments of Periodontology, Aichi-Gakuin University, School of Dentistry

• Hayakawa Taro

  Biochemistry, Aichi-Gakuin University, School of Dentistry

• Noguchi Toshihide

  Departments of Periodontology, Aichi-Gakuin University, School of Dentistry

Bibliographic Information

Other Title

• ＴＩＭＰ‐１およびＴＩＭＰ‐２は破骨細胞形成を促進する

Abstract

Tissue inhibitor of metalloproteinases-1 (TIMP-1) and TIMP-2, originally known as intrinsic inhibitors of matrix metalloproteinases (MMPs), are now known as constitutive components in serum and have potent cell growth-promoting activity. In this study, we examined the effects of TIMPs in FCS on osteoclast-like (OCL) cell formation in mouse bone marrow culture. The OCL cell formation is totally dependent on the presence of PGE₂ or 1α25 (OH)₂D₃ or IL-1α. The effects of these factors were, however, not completely but greatly suppressed by deleting TIMP-1 and TIMP-2 from FCS in culture. But the suppression was almost fully recovered by the readdition of the same amount of recombinant (r) TIMPs which were detected in 10% FCS. As low molecular weight MMP inhibitors could not substitute rTIMPs for the recovery, TIMP activity on the OCL cell formation seemed to be independent from their MMP inhibitory activity. These results strongly suggest a secondary but potent stimulating activity of TIMPs on OCL cell formation. In conclusion, TIMPs in serum play an important role in osteoclastgenesis in mouse bone marrow culture.

Journal

• Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology)

  Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) 47 (4), 297-307, 2005

  JAPANESE SOCIETY OF PERIODONTOLOGY

Keywords

• tissue inhibitors of metalloproteinases (TIMP-1 and TIMP-2)
• osteoclasts
• bone marrow culture

Details

• CRID: 1390001204410513664
• NII Article ID: 10016919077
• NII Book ID: AN0019129X
• DOI: 10.2329/perio.47.297
• ISSN: 1880408X
• ISSN: 03850110
• Web Site: https://search.jamas.or.jp/link/ui/2006083397
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed