クエの精子凍結保存方法と凍結精子を用いた人工受精

  • 今泉 均
    独立行政法人水産総合研究センター奄美栽培漁業センター
  • 堀田 卓朗
    独立行政法人水産総合研究センター古満目栽培漁業センター
  • 太田 博巳
    近畿大学農学部水産学科

書誌事項

タイトル別名
  • Cryopreservation of Kelp Grouper Epinephelus bruneus Sperm and Comparison of Fertility of Fresh and Cryopreserved Sperm
  • クエ ノ セイシ トウケツ ホゾン ホウホウ ト トウケツ セイシ オ モチイタ ジンコウ ジュセイ

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抄録

Since the kelp grouper is the protogynous fish, it is difficult to obtain mature males. We examined a method for the cryopreservation of the sperm for the development of systematic production of the seedlings of the fish. Five aliquots of DMSO (cryoprotectant) plus 93 aliquots of 300 mM glucose (extender) constituted the most suitable milt diluent for cryopreservation compared with other concentrations of DMSO or Glycerol as a cryoprotectant and artificial seminal plasma or 300 mM sucrose as an extender. Milt was diluted at 50 times with the diluent and dispensed into 500μl straws. The straws were suspended at various heights (from 1 to 7 cm) above the liquid surface of nitrogen (LN) for 5 min then immersed into the LN. Straws suspended at 3 cm (the cooling rate was - 32.5°C /min, and the temperature reached was - 144°C ) contained spermatozoa with the highest post-thaw motility. With 20μl of post-thaw milt added to 10 g of eggs, fertility exceeded 80%, and hatchability was equivalent to that of fresh milt. These results indicate that cryopreserved sperm of the kelp grouper is adaptable for the artificial fertilization on a commercial scale.

収録刊行物

  • 水産増殖

    水産増殖 53 (4), 405-411, 2005

    日本水産増殖学会

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