Effects of chitin, chitosan, and oligochitosan on the antimicrobial activity of clarithromycin in combination with rifampicin against Mycobacterium avium complex within mouse peritoneal macrophages

• Sato Katsumasa

  Department of Microbiology and Immunology, Shimane University School of Medicine

• Sano Chiaki

  Department of Microbiology and Immunology, Shimane University School of Medicine

• Shimizu Toshiaki

  Department of Microbiology and Immunology, Shimane University School of Medicine

• Tomioka Haruaki

  Department of Microbiology and Immunology, Shimane University School of Medicine

Bibliographic Information

Other Title

• Ｍｙｃｏｂａｃｔｅｒｉｕｍ　ａｖｉｕｍに対するマクロファージの殺菌能における抗菌薬とキチン・キトサン・キトサンオリゴ糖の併用効果

Abstract

Since Mycobacterium avium complex (MAC) infections are refractory, the development of new drugs with strong anti-MAC activity or therapeutic regimens using ordinary antimycobacterial drugs in combination with immunoadjuncts is urgently desired. In this study, we studied the effects of chitin, chitosan, and oligochitosan, which exhibit immunopotentiating activity, on the antimicrobial activity of clarithromycin (CAM) in combination with rifampicin (RFP) against MAC organisms replicating within murine peritoneal macrophages (MΦS). Murine peptone-induced peritoneal exudate cells were cultured for 3 days with or without chitin, chitosan, or oligochitosan at concentrations of 10 μg/mL to 100μg/mL and washed with 2% FBS-HBSS; the resulting MΦs were then infected with MAC. When MAC-infected cells were cultured in the presence or absence of CAM/RFP at Cmax doses (CAM, 2.3μg/mL; RFP, 6.2μg/mL) for 7 days, CAM/RFP significantly eliminated the intracellular organisms within MΦs. Although chitin, chitosan and oligochitosan alone did not inhibit intramacrophage bacterial growth, chitin and chitosan, but not oligochitosan, potentiated the bactericidal activity of CAM/RFP against intracellular MAC organisms. Next, chitin and chitosan were examined for their effects on the therapeutic activity of CAM/RFP against MAC infection in mice. Chitin and chitosan did not increase the anti-MAC therapeutic activity of CAM/RFP.

Journal

• Japanese Journal of Chemotherapy

  Japanese Journal of Chemotherapy 54 (1), 39-42, 2006

  Japanese Society of Chemotherapy

Keywords

• Mycobacterium avium
• macrophage
• chitin
• chitosan

Details

• CRID: 1390001206285331840
• NII Article ID: 10017235222
• NII Article ID: 130004298285
• NII Book ID: AN10472127
• DOI: 10.11250/chemotherapy1995.54.39
• ISSN: 18845886
• ISSN: 13407007
• Web Site: https://search.jamas.or.jp/link/ui/2006106306
• Text Lang: ja
• Data Source:
  • JaLC
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed