腹部中〓穴(CV12)への鍼通電刺激が経静脈糖負荷試験および Hyperinsulinemic Euglycemic Clamp 時の糖消費に及ぼす効果 Electroacupuncture at the Zhongwan (CV12) Acupoint Accelerates Glucose Consumption during an Intravenous Glucose Tolerance Test and Hyperinsulinemic Euglycemic Clamp in Sprague-Dawley Rats

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Author(s)

    • 石崎 直人 ISHIZAKI Naoto
    • 明治鍼灸大学 臨床鍼灸医学I教室 Department of Clinical Acupuncture and Moxibustion I, Meiji University of Oriental Medicine
    • 矢野 忠 YANO Tadashi
    • 明治鍼灸大学 健康鍼灸医学教室 Department of Health Promotive Acupuncture and Moxibustion, Meiji University of Oriental Medicine

Abstract

腹部への鍼通電刺激がラット空腹時血糖値を降下させる作用が報告されているが、糖負荷時の効果については検討されていない。また同様の刺激がインスリン抵抗性を改善する可能性も示唆されているが、明確に検証されていない。我々は、腹部中〓 (CV12) への鍼通電刺激が、糖負荷時の血糖および hyperinsulinemic euglycemic clamp 時の糖代謝に及ぼす効果について検討した。一晩絶食した雄性SDラットを、pentobarbital で持続麻酔し尾動脈に採血用のカテーテルを挿入した。血糖値が安定した後に採血を行い30分後に2回目の採血を行なった。糖負荷は2回目の採血の後尾静脈に0.5g/kgのブドウ糖を注入して、負荷後2、5、10、20、30、45、60分の時点で血糖値および血漿インスリン値を測定した。鍼刺激は糖負荷前30分間 (EA30 n=8) もしくは糖負荷前および負荷中の90分間行う場合 (EA90 n=8) の2群とし、鍼通電刺激を行わないコントロール群と比較した。hyperinsulinemic euglycemic clamp は、前採血の後、インスリンを持続注入 (2mU/kg/min) し、空腹時の血糖値を維持するようにブドウ糖を注入し、鍼刺激中の変動を観察した。結果、空腹時30分の鍼刺激では血糖値は降下し、インスリン値は上昇した。また糖負荷時の総血糖量はコントロールに比較してEA30およびEA90で有意に低く、相対的インスリン分泌量は鍼通電刺激の群で高かった。hyperinsulinemic euglycemic clamp では、40分間の鍼刺激中にブドウ糖注入率 (GIR) は増加し、インスリン分泌もコントロールと比較して高値であった。インスリン感受性は鍼刺激後半で上昇した。腹部への鍼通電刺激による血糖降下作用にはインスリン感受性亢進およびインスリン分泌亢進が関与することが示唆された。

<b>Background</b><br>Although there are several reports that electroacupuncture (EA) in the abdomen reduces fasting blood glucose and improves insulin sensitivity, the effects under a glucose load or hyperinsulinemic conditions have not yet been studied. In this study, we investigated the effect of EA at Zhongwan (CV12) under the conditions of an intravenous glucose tolerance test (IVGTT) and a hyperinsulinemic euglycemic clamp in normal Sprague-Dawlay (SD) rats.<br><b>Methods</b><br>Male SD rats were anesthetized with pentobarbital (40mg/kg i. p.) and then maintained by continuous infusion through a tail vein. Blood samples were drawn from the ventral tail artery during the fasting stage (baseline and 30min after), and at 2, 5, 10, 20, 30, 45, 60min after a glucose load (0.5g/kg). EA was performed for 30min (EA30, n=8) during the fasting stage and for 90min (EA90, n=8) during both the fasting and IVGTT periods. In the hyperinsulinemic euglycemic clamp experiments, insulin (2mU/kg/min) was infused through the tail vein, followed by infusion of 20% glucose at variable rates to maintain fasting blood glucose levels. EA was performed for 40min after a steady-state was achieved.<br><b>Results</b><br>Significant decreases in fasting blood glucose and increase in plasma insulin concentration were observed during the fasting period in rats in both the EA30 and EA90 groups, whereas rats in the control group (n=8) which did not receive any EA stimulation showed no such changes. Total glucose levels during the IVGTT were lower in the EA30 and EA90 groups compared to controls, with a significantly higher level of relative insulin secretion. During the hyperinsulinemic euglycemic clamp, glucose consumption was increased significantly by EA stimulation with a marked increase in both insulin concentration and sensitivity.<br><b>Conclusion</b><br>EA at CV12 accelerates glucose consumption during IVGTT and hyperinsulinemic conditions probably as a consequence of increased insulin sensitivity and/or increased plasma insulin concentration.

Journal

  • The Journal of The Japanese Society of Balneology, Climatology and Physical Medicine

    The Journal of The Japanese Society of Balneology, Climatology and Physical Medicine 69(2), 109-120, 2006-02-01

    The Japanese Society of Balneology, Climatology and Physical Medicine

References:  31

Codes

  • NII Article ID (NAID)
    10017262507
  • NII NACSIS-CAT ID (NCID)
    AN00186245
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00290343
  • NDL Article ID
    7827266
  • NDL Source Classification
    ZS47(科学技術--医学--治療医学・看護学・漢方医学)
  • NDL Call No.
    Z19-225
  • Data Source
    CJP  NDL  J-STAGE 
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