PCRによる小児口腔内からの歯周病関連細菌の検出  [in Japanese] Detection of Bacteria Related to Periodontal Disease in Oral Cavity of Japanese Children by Species-specific PCR  [in Japanese]

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今回, 我々は, 小児口腔内から<I>Porphyromonas gingivalis (P.g.), Actinobacillus actinomycetemcomitans (A. a.), Prevotella intermedia (P. i.) </I>, および<I>Bacteroides forsythus (B.f.) </I>の4菌種をPCR法で検出し, その検出結果と臨床診査との関係について検討した.被験児は本学小児歯科外来を受診し, 全身的に健康な小児37名とし, 上顎右側中切歯 (FDI11) と上顎右側第一大臼歯 (FDI16) の近心唇側・頬側面から歯肉縁上プラークを採取した.細菌の検出は, プラーク抽出DNAを鋳型とし, 菌種特異的なプライマーを用いたPCR反応により行った.また, 臨床診査は口腔内診査, ProbingDepth, およびBOPの有無について行った.臨床診査結果から対象とした小児37名は, いずれも歯周疾患に罹患していなかった.本研究における歯肉縁上プラーク中の歯周病関連細菌の検出結果では, <I>P.g</I>で21.6%, <I>A.a.</I>, <I>P.i, B.f.</I>, の3菌種では55~58%の検出率であった.年齢別でみると, <I>A.a., P.i, B.f</I>.の検出率は混合歯列期に高く, 永久歯列完成期では低い傾向を示した.また, BOPの有無と検出率との関係では, <I>P.g</I>.ではBOP (-) 群とBOP (+) 群の検出率に差はなかった.、<I>A.a., P.i, B.f</I>.では, BOP (一) 群に比べ, BOP (+) 群の方が高い傾向を示した.本研究では, 6~18歳の小児の歯肉縁上プラーク中に歯周病関連細菌が高頻度に存在したことが示された.また, 歯周疾患の予防の点から将来歯周疾患に罹患するリスクの高い小児を早期にスクリーニングし, 小児期から対処する必要性が示唆された.

The purpose of this study was detect the presence of four putative periodontopathic bacteria Porphyromonas gingivalis (<I>P. g.), Actinobacillus actinomycetemcomitans (A. a.), Prevotella intermedia (P. i.)</I>, and <I>Bacteroides forsythus (B. f)</I> using PCR in the oral cavities of children, then investigated relationships between bacterial species and various clinical parameters.<BR>The subject of 37 children were enrolled the pediatric dental clinic at the Showa University Dental Hospital in Japan. Children with systematic diseases were not excluded, although no subjects who had taken antibiotics within the past 3 month were included. A supragingival plaque sample was collected from the mesiobuccal and labial surfaces of the right maxillary central incisor (FDI 11) and the right maxillary first molar (FDI 16). Extracted DNA from plaque samples was used for PCR in this study. The amplification products were photographed under UV illumination. The following clinical parameters were also assessed : intraoral examination, probing depth (PD) and Bleeding On Probing (BOP).<BR>In this study, detection rate for <I>P. g.</I> was 21.6% and <I>A. a., P. i</I>. and <I>B. f</I> were detected from 55% to 58% in supragingival plaque. All subjects were not periodontitis. In addition, detection rates for <I>A. a., P. i</I>. and <I>B. f</I> were high in children with mixed dentition, but low in those with permanent dentition. Furthermore, regarding the relationship between bacterial species and BOP, it was not regarded between <I>P. g.</I> and BOP. Detection rates of <I>A. a., P. i.</I> and <I>B. f</I> were higher for BOP (+) than for BOP (-).<BR>Detection rates of putative periodontopathic bacteria from healthy children 6-18 years of age was higher in this study. The result suggest that it was need searching of children had a high risk facter for periodontal disease in childhood.


  • The Journal of Showa University Dental Society

    The Journal of Showa University Dental Society 24(2), 187-195, 2004-06-30

    Showa University Dental Society

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