Application of the Nanogold-4,4′-bis(methanethiol)biphenyl Modified Gold Electrode to the Determination of Tyrosinase-Catechol Reaction Kinetics in Acetonitrile

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  • NAKAMURA Toshio
    Department of Chemistry, Faculty of Science, Shinshu University
  • REN Jujie
    Department of Chemistry, Faculty of Science, Shinshu University
  • ZHU Kai-mei
    Department of Chemistry, Faculty of Science, Shinshu University
  • KAWARA Shinshi
    Department of Chemistry, Faculty of Science, Shinshu University
  • JIN Baokang
    Department of Chemistry, Faculty of Science, Shinshu University

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  • Application of the Nanogold-4,4'-bis(methanethiol)biphenyl Modified Gold Electrode to the Determination of Tyrosinase-Catechol Reaction Kinetics in Acetonitrile

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Abstract

The reactivity of tyrosinase adsorbed on nanogold bound with 4,4′-bis(methanethiol)biphenyl monolayer self-assembled on a gold disk with catechol in a dipolar aprotic solvent, acetonitrile (AN), was studied by cyclic voltammetric and amperometric methods. Tyrosinase exhibited characteristics of a Michaelis-Menten kinetic mechanism. The tyrosinase attached to the nanogold continued to react with substrates in AN even when the water content was lower than 0.01 w/w%. The apparent Michaelis-Menten constant Km of tyrosinase for catechol is 5.5 ± 0.4 mM (n = 5).

Journal

  • Analytical Sciences

    Analytical Sciences 22 (9), 1261-1264, 2006

    The Japan Society for Analytical Chemistry

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