Inhibition by Selenium Compounds of Catecholamine Secretion Due to Inhibition of Ca^<2+> Influx in Cultured Bovine Adrenal Chromaffin Cells

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Author(s)

Abstract

Selenium is an essential trace metal element, whereas large doses of selenium exert adverse effects to the human body. We examined the effects of selenium compounds, sodium selenite (Na<sub>2</sub>SeO<sub>3</sub>) and sodium selenate (Na<sub>2</sub>SeO<sub>4</sub>), on catecholamine secretion from cultured bovine adrenal chromaffin cells. Treatment of chromaffin cells with sodium selenite for 72, 48, and 24 h caused decreases in protein and catecholamine contents, in association with cell damage, at concentrations over 30, 300, and 300 μM, respectively. The cells treated with subtoxic conditions (<100 μM, 48 h) of sodium selenite were used for further experiments. Sodium selenite treatment for 48 h inhibited carbachol (CCh)-induced catecholamine secretion in a concentration-dependent and non-competitive manner, while it did not affect high K<sup>+</sup>- and veratridine-induced catecholamine secretion. Sodium selenite (100 μM) did not affect CCh- and veratridine-induced <sup>22</sup>Na<sup>+</sup> influx, while the compound inhibited <sup>45</sup>Ca<sup>2+</sup> influx induced only by CCh, but not high K<sup>+</sup> and veratridine. Sodium selenate even at higher concentrations (1000 μM) did not affect any stimulus-induced catecholamine secretion and <sup>45</sup>Ca<sup>2+</sup> influx. Thus, sodium selenite may specifically exert adverse effects, such as inhibition of physiological stimulus-induced catecholamine secretion from adrenal chromaffin cells due to inhibition of Ca<sup>2+</sup> influx.<br>

Journal

  • Journal of Pharmacological Sciences

    Journal of Pharmacological Sciences 101(3), 223-229, 2006-07-20

    The Japanese Pharmacological Society

References:  24

Codes

  • NII Article ID (NAID)
    10018236954
  • NII NACSIS-CAT ID (NCID)
    AA11806667
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    13478613
  • NDL Article ID
    7985591
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  NDL  J-STAGE 
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