Micro-CT observation of rat dental pulp healing after pulpotomy in <I>in vivo</I> study

• Osuga Naoto

  Department of Pediatric Dentistry, Matsumoto Dental University Department of Oral Health Promotion, Matsumoto Dental University

• Yang Jing

  Department of Hard Tissue Research, Matsumoto Dental University

• Yamakawa Yoko

  Yamakawa Dental Clinic

• Ninomiya Tadashi

  Department of Hard Tissue Research, Matsumoto Dental University

• Arai Yoshinori

  Department of Hard Tissue Research, Matsumoto Dental University

• Raorao Wang

  Department of Pediatric Dentistry, Matsumoto Dental University

• Iwasaki Hiroshi

  Department of Pediatric Dentistry, Matsumoto Dental University Department of Oral Health Promotion, Matsumoto Dental University

• Miyazawa Hiroo

  Department of Pediatric Dentistry, Matsumoto Dental University Department of Oral Health Promotion, Matsumoto Dental University

Abstract

We report the newly developed Micro-CT, which allows us to observe the individual animal over a long experimental period and to compare changes in pulp tissue in relation to growth and aging without considering individual differences. Further, we used pathological examination to prove similar the result observing from Micro-CT. We have examined wound healing of teeth after pulpotomy in rats, and could clearly observe histopathological changes in the affected teeth and the absorption of temporary filling material and pulp capping agents. In cases with breakage of the dental crown, the CT images agreed with the pathological observations, and it was possible to estimate the time of breakage. In vivo Micro-CT is possible to apply in continuous recording of small experimental animals, such as rats, under anesthesia and the result is sufficiently high. High-quality image was obtained in of the entire head region of the rat. It was suggested that this method can be used for long-term continuous observation of changes in the teeth conditions after pulpotomy in experimental animals. We report the newly developed Micro-CT, which allows us to observe the individual animal over a long experimental period and to compare changes in pulp tissue in relation to growth and aging without considering individual differences.

Journal

• Pediatric Dental Journal

  Pediatric Dental Journal 16 (2), 132-137, 2006

  The Japanese Society of Pediatric Dentistry

Keywords

• Calcium hydroxide
• Formocresol
• Micro-CT
• Pulp tissue
• Pulpotomy

Details

• CRID: 1390001204384243200
• NII Article ID: 130004475800
• NII Article ID: 10018252765
• NII Book ID: AA10809637
• DOI: 10.11411/pdj.16.132
• ISSN: 18803997
• ISSN: 09172394
• Web Site: https://search.jamas.or.jp/link/ui/2007115466
• Text Lang: en
• Data Source:
  • JaLC
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed