Assessment of Fixed Charge Density in Regenerated Cartilage by Gd-DTPA-enhanced MRI
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- MIYATA Shogo
- Graduate School of Life Science and System Engineering, Kyushu Institute of Technology
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- HOMMA Kazuhiro
- National Institute of Advanced Industrial Science and Technology (AIST)
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- NUMANO Tomokazu
- National Institute of Advanced Industrial Science and Technology (AIST)
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- FURUKAWA Katsuko
- Graduate School of Engineering, University of Tokyo
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- TATEISHI Tetsuya
- National Institute for Material Science
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- USHIDA Takashi
- Graduate School of Medicine, University of Tokyo
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Abstract
Purpose: Applying regenerated cartilage in a clinical setting requires noninvasive evaluation to detect the maturity of cartilage tissue. Magnetic resonance (MR) imaging of articular cartilage is well accepted and has been applied clinically in recent years. We attempt to establish a noninvasive method to evaluate the maturity of regenerated cartilage tissue using gadolinium-enhanced MR imaging.<br> Methods: To reconstruct cartilaginous tissue, we embedded articular chondrocytes harvested from bovine humeral head in agarose gel and cultured the cells in vitro up to 4 weeks. The fixed charge density (FCD) of the cartilage was determined using MRI gadolinium exclusion method. The sulfated glycosaminoglycan (sGAG) content was determined by dimethylmethylene blue dye-binding assay.<br> Results: The sGAG content and FCD of the regenerated cartilage increased with duration of culture. In the T1Gd maps, the [Gd-DTPA2-] in the specimen decreased, and the boundary between the sample disk and the bath solution of phosphate buffered saline (PBS) became clearer as time in culture increased. In the linear regression analysis, FCD and sGAG content correlated significantly.<br> Conclusion: Gadolinium-enhanced MR imaging measurements can be useful predictors of the degree of cartilaginous tissue formation.<br>
Journal
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- Magnetic Resonance in Medical Sciences
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Magnetic Resonance in Medical Sciences 5 (2), 73-78, 2006
Japanese Society for Magnetic Resonance in Medicine
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Keywords
Details 詳細情報について
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- CRID
- 1390001205194920960
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- NII Article ID
- 10018258510
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- NII Book ID
- AA11648770
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- ISSN
- 18802206
- 13473182
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed