CLOCK/BMAL1 is Involved in Lipid Metabolism via Transactivation of the Peroxisome Proliferator-activated Receptor (PPAR) Response Element

• Inoue Ikuo

  Fourth Department of Medicine, Research Center for Genomic Medicine, Saitama Medical School, Saitama, Japan.

• Shinoda Yuichi

  Department of Biochemistry, Research Center for Genomic Medicine, Saitama Medical School, Saitama, Japan.

• Ikeda Masaaki

  Department of Physiology, Research Center for Genomic Medicine, Saitama Medical School, Saitama, Japan. Molecular Clock Project, Research Center for Genomic Medicine, Saitama Medical School, Saitama, Japan.

• Hayashi Kenji

  Fourth Department of Medicine, Research Center for Genomic Medicine, Saitama Medical School, Saitama, Japan.

• Kanazawa Kenta

  Fourth Department of Medicine, Research Center for Genomic Medicine, Saitama Medical School, Saitama, Japan.

• Nomura Masahiko

  Department of Physiology, Research Center for Genomic Medicine, Saitama Medical School, Saitama, Japan.

• Matsunaga Toshiyuki

  Department of Biochemistry, Research Center for Genomic Medicine, Saitama Medical School, Saitama, Japan.

• Xu Haiyuan

  Fourth Department of Medicine, Research Center for Genomic Medicine, Saitama Medical School, Saitama, Japan. Department of Physiology, Research Center for Genomic Medicine, Saitama Medical School, Saitama, Japan. Molecular Clock Project, Research Center for Genomic Medicine, Saitama Medical School, Saitama, Japan.

• Kawai Shinichiro

  Department of Biochemistry, Research Center for Genomic Medicine, Saitama Medical School, Saitama, Japan.

• Awata Takuya

  Fourth Department of Medicine, Research Center for Genomic Medicine, Saitama Medical School, Saitama, Japan.

• Komoda Tsugikazu

  Department of Biochemistry, Research Center for Genomic Medicine, Saitama Medical School, Saitama, Japan.

• Katayama Shigehiro

  Fourth Department of Medicine, Research Center for Genomic Medicine, Saitama Medical School, Saitama, Japan.

抄録

Lipid absorption and metabolism are regulated by feeding and by the circadian system. It has been suggested that the expression of enzymes involved in lipid metabolism is directly controlled by the clock system. This study was designed to examine whether or not the CLOCK/BMAL1 heterodimer has transcriptional activity for genes via the peroxisome proliferator-activated receptor response element (PPRE). Male mice 8-12 weeks old were maintained under a 12:12 hour light-dark cycle for at least two weeks before the day of the experiment. The mRNA profiles of BMAL1 and of the PPAR target genes acyl-CoA oxidase (AOX), 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase and cellular retinol binding protein II</B> (CRBPII</B>) were measured in intestine. The direct effects of CLOCK/BMAL1 on the promoter activities of those three enzymes were assessed in vitro by luciferase assay. The expression of PPAR target genes changed in a cyclical manner that followed expression of BMAL1. The promoter activities of the three enzymes were increased by CLOCK/BMAL1 expression. After deletion of the PPRE from the CRBPII</B> construct, CLOCK/BMAL1 did not affect transactivation. CLOCK/BMAL1 transactivates PPAR target genes via the PPRE.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ａｔｈｅｒｏｓｃｌｅｒｏｓｉｓ　ａｎｄ　Ｔｈｒｏｍｂｏｓｉｓ

  Ｊｏｕｒｎａｌ　ｏｆ　Ａｔｈｅｒｏｓｃｌｅｒｏｓｉｓ　ａｎｄ　Ｔｈｒｏｍｂｏｓｉｓ 12 (3), 169-174, 2005

  一般社団法人 日本動脈硬化学会

キーワード

• CLOCK
• BMAL1
• PPARα
• acyl-CoA oxidase
• HMG-CoA synthase
• Cellular retinol binding protein II (CRBP II)

詳細情報

• CRID: 1390001204431293312
• NII論文ID: 10018365876
• NII書誌ID: AA11018976
• DOI: 10.5551/jat.12.169
• COI: 1:CAS:528:DC%2BD28XnvVyrtr4%3D
• ISSN: 18803873
• ISSN: 13403478
• ISSN: http://id.crossref.org/issn/13403478
• PubMed: 16020918
• Web Site: https://www.jstage.jst.go.jp/article/jat/12/3/12_3_169/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2006163208
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可