Plasma proteomic analysis using liposome as a biological ligand.

• Kimura Michitoshi

  Department of Biomedical Engineering, Sapporo Medical University School of Medicine

• Sohma Hitoshi

  Department of Biomedical Engineering, Sapporo Medical University School of Medicine

• Naishiro Yasuyoshi

  Department of Biomedical Engineering, Sapporo Medical University School of Medicine

• Kokai Yasuo

  Department of Biomedical Engineering, Sapporo Medical University School of Medicine

Bibliographic Information

Other Title

• リポソームをリガンドとして用いた血漿蛋白質のプロテオミクス解析
• リボソーム オ リガンド ト シテ モチイタ ケッショウ タンパクシツ ノ プロテオミクス カイセキ

Abstract

Human plasma contains a numerous number of proteins that provide variable information reflecting to physiological and pathological conditions of human body. Some of such proteins have been expected for pathobiological markers for diagnosis and drug development for human diseases. To capture plasma proteins in a manner of high-through put, several ligands have been employed, though these systems are still required many improvements. In this report, we present a novel system using liposome as a ligand to capture a wide variety of plasma proteins. One hundred micro litter of plasma was incubated with liposome of multi-lamellar vesicle in the presence of Ca²⁺. Proteins bound to liposome were then eluted in the presence of EGTA. A two-dimensional gel analysis revealed more than 150 proteins in one electrophoresis run in a range of pH 4-7 with a molecular weight between 100kda to 30kda. The pattern of silver-stained proteins showed reproducible with the same donor. Apparent differences were detected between the pattern under different pathologic conditions including healthy volunteer and that with patients with chronic inflammation. Taken together, the method using liposome might provide a useful biological ligand for plasma protein analysis and a novel system for proteomic approach combined with other chromatographic module.

Journal

• SEIBUTSU BUTSURI KAGAKU

  SEIBUTSU BUTSURI KAGAKU 50 (4), 231-236, 2006

  Japanese Electrophoresis Society

Keywords

• liposome
• biological ligand
• plasma
• proteomics

Details

• CRID: 1390282679178132736
• NII Article ID: 130003607386
• NII Article ID: 10018441612
• NII Book ID: AN00129729
• DOI: 10.2198/sbk.50.231
• COI: 1:CAS:528:DC%2BD2sXhtVeitbY%3D
• ISSN: 13499785
• ISSN: 00319082
• ISSN: http://id.crossref.org/issn/00319082
• NDL BIB ID: 8615640
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8615640
• Web Site: https://search.jamas.or.jp/link/ui/2007109286
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed