Determination of Eight Organic Acids in Sake and Wine by Suppressed Ion Chromatography with Triacontyl Groups (C_<30>) as a Stationary Phase
A suppressed ion method for the determination method of eight organic acids (formic, malonic, lactic, acetic, malefic, citric, fumaric, and propionic acid) has been studied for reversed-phase high-performance liquid chromatography (RP-HPLC) with a new triacontyl groups (C<SUB>30</SUB>) column, coupled with an ultraviolet detector (UV) at 210 nm. A 50 mM ammonium dihydrogenphosphate (NH<SUB>4</SUB>H<SUB>2</SUB>PO<SUB>4</SUB>) aqueous solution (pH 2.0) containing phosphoric acid (H<SUB>3</SUB>PO<SUB>4</SUB>) was used as an optimal mobile phase for the separation of eight organic acids. A good linearity of the calibration curve was obtained in the concentration range of 5 mgl<SUP>-1</SUP> to 100 mgl<SUP>-1</SUP> (|r| =0.9999-0.9994) . The detection limit based on <I>S</I>/<I>N</I>=3 were 3.8 mgl<SUP>-1</SUP> for formic acid, 3.5 mgl<SUP>-1</SUP> for malonic acid, 3.1 mgl<SUP>-1</SUP> for lactic acid, 3.4 mgl<SUP>-1</SUP> for acetic acid, 1.1 mgl<SUP>-1</SUP> for maleic acid, 3.4 mgl<SUP>-1</SUP> for citric acid, 3.3 mgl<SUP>-1</SUP> for fumaric acid, and 3.6 mgl<SUP>-1</SUP> for propionic acid. This analytical method was successfully applied to the determination of eight organic acids in sake and wine.
- 環境化学 : journal of environmental chemistry
環境化学 : journal of environmental chemistry 16(4), 691-696, 2006-12-25
Japan Society for Environmental Chemistry