Development of Two Stages Cock's Semen Extender for Room Temperature Storage at Laboratory
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- Ameha Negassi
- Department of Animal Science, Alemaya University
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- Moudgal Ram P.
- Department of Animal Science, Alemaya University
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- Asmare Asefa
- Department of Animal Science, Alemaya University
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抄録
A simple cock’s semen dilutor (two-stage) with minimum ingredients was developed in the present study. The stage-1dilutor was aimed to reduce the motility and metabolism during storage and the stage-2 dilutor was to be added just prior to insemination to revive the motility to facilitate the entry of spermatozoa into sperm host gland. Initially, the semen was diluted 1 : 1 with the stage-1 dilutor having lower pH that reduced the motility and metabolism. Subsequently, after 24h, the diluted semen was mixed slowly with another dilutor stage-2 at 1 : 2 ratios to revive the motility of the spermatozoa to normal level. Phosphate buffer was the base for both storage (stage-1) and recovery (stage-2) dilutors with a pH of 6.5 and 7.5, respectively. In stage-1 dilutor sodium glutamate to minimize metabolism of spermatozoa and antibiotics to check bacterial growth were used whereas these were absent in stage-2 dilutor. Glucose was added as energy source in the dilutor of both stages. The osmolarity of stage-1 and stage-2 dilutors was 346 and 327Osm/kg H20, respectively. This dilutor can be used to store the cock’s semen at room temperature (17-21°C) for 24h with sustained motility. This new approach for two-stage dilutor of cock’s semen storage at room temperature for extended period could be a useful A.I. tool under farm condition.
収録刊行物
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- The Journal of Poultry Science
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The Journal of Poultry Science 44 (1), 78-84, 2007
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詳細情報 詳細情報について
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- CRID
- 1390001205206809088
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- NII論文ID
- 10018485678
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- NII書誌ID
- AA11564513
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- ISSN
- 13490486
- 13467395
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- NDL書誌ID
- 8610996
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
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- 使用不可