Incorporation of Capillary-Like Structures into Dermal Cell Sheets Constructed by Magnetic Force-Based Tissue Engineering
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- Ino Kosuke
- Department of Biotechnology, School of Engineering, Nagoya University Reseach Fellow of the Japan Society for the Promotion of Science (JSPS Research Fellow)
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- Ito Akira
- Department of Chemical Engineering, Faculty of Engineering, Kyushu University
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- Kumazawa Hirohito
- Department of Biotechnology, School of Engineering, Nagoya University
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- Kagami Hideaki
- Department of Tissue Engineering, School of Medicine, Nagoya University
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- Ueda Minoru
- Department of Oral and Maxillofacial Surgery, School of Medicine, Nagoya University
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- Honda Hiroyuki
- Department of Biotechnology, School of Engineering, Nagoya University
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抄録
One of the major challenges in tissue engineering remains the construction of vascularized 3D transplants in vitro. We recently proposed novel technologies, termed “magnetic force-based tissue engineering” (Mag-TE), to establish three-dimensional (3D) tissues without using scaffolds. Magnetite cationic liposomes (MCLs), which contain 10-nm magnetite nanoparticles in order to improve accumulation of magnetite nanoparticles in target cells, were used to magnetically label normal human dermal fibroblasts (NHDFs). Magnetically labeled NHDFs were seeded onto ultralow-attachment plates. When a magnet was placed under the plate, cells accumulate on the bottom of the well. After a 24-h-incubation period, the cells form a sheet-like structure, which contains the major dermal extracellular matrix (ECM) components (fibronectin and type I collagen) within the NHDF sheet. Human umbilical vein endothelial cells (HUVECs) were co-cultured with NHDF sheets by two methods: HUVECs and NHDFs were mixed and then allowed to form cell sheets by Mag-TE; or NHDF sheets were constructed by Mag-TE and HUVECs were subsequently seeded onto NHDF sheets. These methods gave tube-like formation of HAECs, resembling early capillaries, within or on the surface NHDF sheets after short-term 3D co-culture, thus suggesting that Mag-TE may be useful for constructing 3D-tissue involving capillaries.
収録刊行物
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- JOURNAL OF CHEMICAL ENGINEERING OF JAPAN
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JOURNAL OF CHEMICAL ENGINEERING OF JAPAN 40 (1), 51-58, 2007
公益社団法人 化学工学会
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詳細情報 詳細情報について
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- CRID
- 1390001204568239488
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- NII論文ID
- 10018500893
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- NII書誌ID
- AA00709658
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- ISSN
- 18811299
- 00219592
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- NDL書誌ID
- 8612716
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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