Enzymatic Preparation of Metabolic Intermediates, 3-Dehydroquinate and 3-Dehydroshikimate, in the Shikimate Pathway
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- ADACHI Osao
- Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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- ANO Yoshitaka
- Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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- TOYAMA Hirohide
- Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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- MATSUSHITA Kazunobu
- Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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抄録
A method for enzymatic preparation of 3-dehydroquinate and 3-dehydroshikimate in the shikimate pathway was established by controlling the enzyme activity of 3-dehydroquinate dehydratase. When quinate was incubated with the membrane fraction of acetic acid bacteria at pH 5.0, 3-dehydroquinate was formed as the predominant product. 3-Dehydroshikimate was the sole product when incubated at pH 8.0. Mutual separation of the metabolic intermediates was also exemplified.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 70 (12), 3081-3083, 2006
公益社団法人 日本農芸化学会
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詳細情報
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- CRID
- 1390001206477107456
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- NII論文ID
- 10018524354
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD2sXktVKisw%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 8597068
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- PubMed
- 17151445
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可