Characterization of the Nitrate-Nitrite Transporter of the Major Facilitator Superfamily (the nrtP Gene Product) from the Cyanobacterium Nostoc punctiforme Strain ATCC 29133

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Abstract

The products of the <I>NpR1527</I> and <I>NpR1526</I> genes of the filamentous, diazotrophic, fresh-water cyanobacterium <I>Nostoc punctiforme</I> strain ATCC 29133 were identified as a nitrate transporter (NRT) and nitrate reductase (NR) respectively, by complementation of nitrate assimilation mutants of the cyanobacterium <I>Synechococcus elongatus</I> strain PCC 7942. While other fresh-water cyanobacteria, including <I>S. elongatus,</I> have an ATP-binding cassette (ABC)-type NRT, the NRT of <I>N. punctiforme</I> belongs to the major facilitator superfamily, being orthologous to the one found in marine cyanobacteria (NrtP). Unlike the ABC-type NRT, which transports both nitrate and nitrite with high affinity, <I>Nostoc</I> NrtP transported nitrate preferentially over nitrite. NrtP was distinct from ABC-type NRT also in its insensitivity to ammonium-promoted regulation at the post-translational level. The nitrate reductase of <I>N. punctiforme</I> was, on the other hand, inhibited upon addition of ammonium to medium, lending ammonium sensitivity to nitrate assimilation.

Journal

  • Bioscience, Biotechnology, and Biochemistry

    Bioscience, Biotechnology, and Biochemistry 70(11), 2682-2689, 2006-11-23

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

References:  40

Codes

  • NII Article ID (NAID)
    10018524830
  • NII NACSIS-CAT ID (NCID)
    AA10824164
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    09168451
  • NDL Article ID
    8560456
  • NDL Source Classification
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL Call No.
    Z53-G223
  • Data Source
    CJP  NDL  J-STAGE 
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