Oxygenases and Dehalogenases: Molecular Approaches to Efficient Degradation of Chlorinated Environmental Pollutants

• FURUKAWA Kensuke

  Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University

Bibliographic Information

Other Title

• Oxygenases and dehalogenases: molecular approaches to efficient degradaiton of chlorinated environmental pollutants

Abstract

Microbial oxygenases and dehalogenases are key enzymes in the degradation of highly chlorinated compounds, which often become significant environmental pollutants. Oxygenases engineered by the methods of directed evolution exhibit enhanced degradation of PCBs and other chlorinated solvents such as trichloroethene and pentachloroethane. Dehalorespiration is an efficient dechlorination mechanism that is coupled with energy-yielding phosphorylation. Recently, a variety of chloroethene-dehalorespiring anaerobes have been isolated, and their reductive dehalogenases have been characterized in biochemical and genetic bases. This review describes our recent studies on dioxygenases and reductive dehalogenases.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 70 (10), 2335-2348, 2006

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• dioxygenase
• reductive dehalogenase
• directed evolution
• hybrid enzyme
• dehalorespiration

Details

• CRID: 1390282681453863296
• NII Article ID: 10018525300
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.60218
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 8523661
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8523661
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.60218
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed