Temperature Adaptation of Bacillus subtilis by Chromosomal groEL Replacement
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- ENDO Ayako
- Laboratory of Molecular Microbiology, College of Agriculture, Ibaraki University
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- SASAKI Mayumi
- Laboratory of Molecular Microbiology, College of Agriculture, Ibaraki University
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- MARUYAMA Akihiko
- National Institute of Advanced Industrial Science and Technology (AIST)
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- KURUSU Yasurou
- Laboratory of Molecular Microbiology, College of Agriculture, Ibaraki University
Bibliographic Information
- Other Title
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- Temperature Adaptation of<i>Bacillus subtilis</i>by Chromosomal<i>groEL</i>Replacement
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Abstract
We investigated a temperature adaptation of Bacillus subtilis 168 in which chromosomal groEL was replaced with a psychrophilic groEL. This strain can grow at 50 °C but not at 51 °C, a temperature at which wild-type B. subtilis can grow. Using in vivo random mutagenesis by the B. subtilis mutator strain (mutS, mutM, mutY), two thermo-adaptants were isolated from the groEL substituted strain at 52 °C. They contained novel amino acid alterations in their ATP binding motif (T93I) and the inter-monomer contact (R285H) region of GroEL. These results suggest that GroEL participates in bacterial temperature adaptation.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 70 (10), 2357-2362, 2006
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390001206477162752
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- NII Article ID
- 10018525451
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- NII Book ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 8523676
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed