Expression Profile of Amylolytic Genes in Aspergillus nidulans

• NAKAMURA Takashi

  Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University

• MAEDA Yuki

  Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University

• TANOUE Nami

  Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University

• MAKITA Tomohiro

  Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University

• KATO Masashi

  Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University

• KOBAYASHI Tetsuo

  Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University

Bibliographic Information

Other Title

• Expression Profile of Amylolytic Genes in<i>Aspergillus nidulans</i>

Abstract

Aspergillus nidulans possessed 16 putative amylolytic genes consisting of 7 α-glucosidase (agdA-F), 7 α-amylase (amyA-F), and 2 glucoamylase (glaA and B) genes on the genome. Among them, the agdA, agdB, agdE, agdF, amyA, amyB, amyF, and glaB genes were induced by isomaltose. AmyR, a Zn(II)₂Cys₆ transcription factor, was required for the induction. The isomaltose-inducible genes possessed at least one consensus sequence for AmyR binding, 5′-CGGN₈CGG, on each promoter region. None of the amylolytic genes was induced by maltose.<BR>The mRNA levels of the amylolytic genes except for agdC, amyD, and amyG increased under carbon-starved conditions. Release from CreA-dependent carbon catabolite repression was the main cause of the increase, but, the mRNA levels of agdB, agdF, amyB, amyF, and glaB increased to some extent even in a creA mutant. Therefore, both CreA-dependent and -independent mechanisms are involved in the up-regulation of the amylolytic genes under carbon-starved conditions.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 70 (10), 2363-2370, 2006

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• amylase
• <I>Aspergillus nidulans</I>
• AmyR
• CreA
• carbon starvation

Details

• CRID: 1390282681453875840
• NII Article ID: 10018525470
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.50694
• COI: 1:CAS:528:DC%2BD28XhtFynu7%2FN
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 8523689
• PubMed: 17031028
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8523689
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.50694
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed