Isolation and Characterization of an Alcohol Dehydrogenase Gene from the Octylphenol Polyethoxylate Degrader Pseudomonas putida S-5

• TASAKI Yuji

  Department of Materials Engineering, Nagaoka National College of Technology

• YOSHIKAWA Hiromichi

  Department of Life, Environment and Materials Science, Faculty of Engineering, Fukuoka Institute of Technology

• TAMURA Hiroto

  Department of Environmental Bioscience, Faculty of Agriculture, Meijo University

Bibliographic Information

Other Title

• Isolation and Characterization of an Alcohol Dehydrogenase Gene from the Octylphenol Polyethoxylate Degrader<i>Pseudomonas putida</i>S-5

Abstract

Octylphenol polyethoxylate (OPEO_n) biodegradation by Pseudomonas putida S-5 under aerobic conditions is initiated by the oxidation of its terminal alcohol group by alcohol dehydrogenase. A DNA fragment, containing an alcohol dehydrogenase gene (adh1), was isolated using a combination of degenerate PCR and inverse PCR. The predicted translation product of adh1 showed significant sequence similarity to bacterial alcohol dehydrogenases. Furthermore, a flavin-binding motif and signature patterns conserved in type III FAD-dependent alcohol oxidases were detected. Two open reading frames (ORFs) were found upstream of adh1, encoding a putative acyl-CoA synthetase and a putative esterase. Downstream of adh1 and located on the opposite strand was an ORF encoding a putative aldehyde dehydrogenase. Transcription analysis using RT-PCR showed that adh1 is cotranscribed with the putative acyl-CoA synthetase and esterase genes during growth on OPEO_n. ADH1 overproduced in Escherichia coli exhibited activity not only toward various alcohols, including OPEO_ns, but also toward primary aliphatic and aromatic aldehydes.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 70 (8), 1855-1863, 2006

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• alcohol dehydrogenase
• alkylphenol polyethoxylate
• biodegradation mechanism
• octylphenol polyethoxylate
• <I>Pseudomonas putida</I>

Details

• CRID: 1390282681456266112
• NII Article ID: 10018527959
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.60009
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 8040150
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8040150
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.60009
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed