Microbial Production of Optically Active β-Phenylalanine through Stereoselective Degradation of Racemicβ-Phenylalanine

DOI Web Site Web Site 被引用文献1件 参考文献26件
  • MANO Junichi
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
  • OGAWA Jun
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
  • SHIMIZU Sakayu
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

書誌事項

タイトル別名
  • Microbial Production of Optically Active .BETA.-Phenylalanine through Stereoselective Degradation of Racemic .BETA.-Phenylalanine
  • Microbial Production of Optically Active ベータ Phenylalanine through Stereoselective Degradation of Racemic ベータ Phenylalanine

この論文をさがす

抄録

The ability to produce (R)- or (S)-β-phenylalanine from racemic β-phenylalanine through stereoselective degradation was screened for. Variovorax sp. JH2 and Arthrobacter sp. the faculty of Agriculture, Kyoto University (AKU) 638 were found to be potential catalysts for (R)- and (S)-β-phenylalanine production respectively. On 192 h cultivation of Variovorax sp. in medium containing 1.0% (w/v) racemic β-phenylalanine, 0.46% (w/v) (R)-β-phenylalanine with an enantiomeric purity of 99% e.e. was obtained. The initial step of the (S)-isomer degradation was stereoselective transamination. On 312 h cultivation of Arthrobacter sp. in medium containing 1.0% (w/v) racemic β-phenylalanine, 0.51% (w/v) (R)-β-phenylalanine with an enantiomeric purity of 90% e.e. was obtained. The initial step of the (R)-isomer degradation was supposed to be oxidative deamination. Resting cell reaction with vigorous shaking, with cells of Arthrobacter sp. as the catalyst, resulted in production of 0.49% (w/v) of (S)-β-phenylalanine with an enantiomeric purity of 99% e.e. from 1.0% (w/v) racemic β-phenylalanine in 45 h.

収録刊行物

被引用文献 (1)*注記

もっと見る

参考文献 (26)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ