Characterization of a rice gene family encoding type-A diterpene cyclase

• KANNO Yuri

  Department of Bioresource Engineering, Yamagata University

• OTOMO Kazuko

  Department of Bioresource Engineering, Yamagata University

• KENMOKU Hiromichi

  Department of Bioresource Engineering, Yamagata University

• MITSUHASHI Wataru

  Department of Bioresource Engineering, Yamagata University

• YAMANE Hisakazu

  Biotechnology Research Center, University of Tokyo

• OIKAWA Hideaki

  Division of Chemistry, Graduate School of Science, Hokkaido University

• TOSHIMA Hiroaki

  Department of Bioresource Science, College of Agriculture, Ibaraki University

• MATSUOKA Makoto

  Bioscience and Biotechnology Center, Nagoya University

• SASSA Takeshi

  Department of Bioresource Engineering, Yamagata University

• TOYOMASU Tomonobu

  Department of Bioresource Engineering, Yamagata University

書誌事項

タイトル別名

• Characterization of a Rice Gene Family Encoding Type-A Diterpene Cyclases

抄録

We have previously isolated and characterized the rice (Oryza sativa) cDNAs, OsCyc1/OsCPS4, OsCyc2/OsCPS2, OsKS4, OsDTC1/OsKS7, OsDTC2/OsKS8 and OsKS10, which encode cyclases that are responsible for diterpene phytoalexin biosynthesis. Among the other members of this gene family, OsCPS1 and OsKS1 have been suggested as being responsible for gibberellin biosynthesis, OsKSL11 has recently been shown to encode stemodene synthase, and the functions of the three other diterpene cyclase genes in the rice genome, OsKS3, OsKS5 and OsKS6, have not yet been determined. In this study, we show that recombinant OsKS5 and OsKS6 expressed in E. coli converted ent-copalyl diphosphate into ent-pimara-8(14),15-diene and ent-kaur-15-ene, respectively. Neither product is a hydrocarbon precursor required in the biosynthesis of either gibberellins or phytoalexins. OsKS3 may be a pseudogene from which the translated product is a truncated enzyme. These results suggest that the diterpene cyclase genes responsible for gibberellin and phytoalexin biosynthesis are not functionally redundant.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 70 (7), 1702-1710, 2006

  公益社団法人 日本農芸化学会

キーワード

• biosynthesis
• cyclase
• diterpene
• rice

詳細情報

• CRID: 1390001206479873152
• NII論文ID: 10018529282
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.60044
• COI: 1:CAS:528:DC%2BD28Xot1Kmtbk%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 7998014
• PubMed: 16861806
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7998014
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.60044
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
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