Assembly of Staphylococcal Leukocidin into a Pore-Forming Oligomer on Detergent-Resistant Membrane Microdomains, Lipid Rafts, in Human Polymorphonuclear Leukocytes

• NISHIYAMA Akihito

  Laboratory of Applied Microbiology, Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University

• KANEKO Jun

  Laboratory of Applied Microbiology, Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University

• HARATA Masahiko

  Laboratory of Molecular Biology, Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University

• KAMIO Yoshiyuki

  Laboratory of Applied Microbiology, Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University

Abstract

Staphylococcal leukocidin (Luk) consists of LukS and LukF, which cooperatively lyse human polymorphonuclear leukocytes (HPMNLs), monocytes, and macrophages. Here we found that LukS and LukF assembles into hetero-oligomeric pore complexes on the detergent-resistant membrane microdomains, lipid rafts of HPMNLs. When HPMNLs were treated with LukS alone, 24% of the added LukS was localized in lipid rafts. Furthermore, in HPMNLs treated with both LukS and LukF simultaneously, about 90% of high molecular-mass complexes of 100 kDa, which consists of LukS and LukF, were detected in the lipid raft fractions. In contrast, in HPMNLs treated with LukF alone, LukF was not localized in lipid rafts despite binding to the target cell membranes. Ten mM methyl-β-cyclodextrin, a dysfunctioning agent of lipid rafts, completely inhibited assembly of Luk on lipid rafts, and resulted in null leukocytolytic activity of Luk. Hence, we concluded that assembly of LukS and LukF into the pore-complex occurs in lipid rafts in HPMNLs and that LukF can bind to LukS, which had already bound to lipid rafts, to assemble into hetero-oligomers.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 70 (6), 1300-1307, 2006

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• staphylococcal leukocidin
• LukS
• pore-forming toxin
• assembly of toxin components
• detergent-resistant membrane microdomains, lipid rafts

Details

• CRID: 1390282681456418176
• NII Article ID: 10018529746
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.50499
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 7958719
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7958719
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.50499
• Web Site: https://search.jamas.or.jp/link/ui/2007094658
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed