Enzymatic Production of Highly Soluble Myricitrin Glycosides Using β-Galactosidase

• SHIMIZU Ryosuke

  Fundamental Research Division, San-Ei Gen F.F.I. Corporation

• SHIMABAYASHI Hiroshi

  Fundamental Research Division, San-Ei Gen F.F.I. Corporation

• MORIWAKI Masamitsu

  Fundamental Research Division, San-Ei Gen F.F.I. Corporation

書誌事項

タイトル別名

• Enzymatic Production of Highly Soluble Myricitrin Glycosides Using ベータ Galactosidase

抄録

Myricitrin, a botanical flavonol glycoside, could be a useful ingredient of functional foods, cosmetics, and medicines because of its high anti-oxidative activity. However, due to its insolubility in water, it has a limited range of use. To improve this solubility, we glycosylated myricitrin by an enzymatic transglycosylate reaction. Myricitrin was galactosylated by β-galactosidase from Bacillus circulans using lactose as a sugar donor. The reaction product was 480 times more soluble than myricitrin. Four myricitrin galactosides were isolated from the reaction products by column chromatography, and their molecular structures were identified by using ESI-MS, ¹H-NMR, ¹³C-NMR, ¹H–¹H COSY, ¹H-¹³C HMQC and ¹H-¹³C HMBC analysis. The solubility of these four myricitrin galactosides was more than 3.9×10³ fold that of myricitrin, and each had similar anti-oxidative activity to that of myricitrin.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 70 (4), 940-948, 2006

  公益社団法人 日本農芸化学会

キーワード

• myricitrin
• β-galactosidase
• transglycosylation
• water solubility
• anti-oxidative activity

詳細情報

• CRID: 1390282681453281152
• NII論文ID: 10018532524
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.70.940
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 7899900
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7899900
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.70.940
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • CiNii Articles
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