Immunochemical Analysis of Aquaporin Isoforms in Arabidopsis Suspension-Cultured Cells

• KOBAE Yoshihiro

  Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences, Nagoya University

• MIZUTANI Masahiro

  Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences, Nagoya University

• SEGAMI Shoji

  Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences, Nagoya University

• MAESHIMA Masayoshi

  Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences, Nagoya University

Bibliographic Information

Other Title

• Immunochemical Analysis of Aquaporin Isoforms in<i>Arabidopsis</i>Suspension-Cultured Cells

Abstract

Aquaporins mediate the movement of water across biomembranes. Arabidopsis thaliana contains 35 aquaporins that belong to four subfamilies (PIP, TIP, SIP, and NIP). We investigated their expression profiles immunochemically in suspension-cultured Arabidopsis thaliana cells during growth and in response to salt and osmotic stresses. Protein amounts of all aquaporins were much lower in cultured cells than in the plant tissues. This is consistent with the low water permeability of protoplasts from cultured cells. After treatment with NaCl, the protein amounts of PIP2;1, PIP2;2, and PIP2;3 in the cells increased several-fold, and those of TIP1;1 and TIP1;2, 15- and 3-fold respectively. PIP1 did not change under the stress. Cell death began after 19 d in culture, accompanied by marked accumulation of PIPs and TIPs and a gradual decrease in SIPs. Our results suggest the followings: (i) Accumulation of aquaporin isoforms was individually regulated at low levels in single cells. (ii) At least PIP2;2, PIP2;3, TIP1;1, and TIP1;2 are stress-responsive aquaporins in suspension cells. (iii) A sudden increment of several members of PIP2 and TIP1 subfamilies might be related to cell death.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 70 (4), 980-987, 2006

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• aquaporin
• <I>Arabidopsis thaliana</I>
• cell death
• stress response
• suspension-cultured cell

Details

• CRID: 1390001206476539520
• NII Article ID: 10018532678
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.70.980
• ISSN: 13476947
• ISSN: 09168451
• ISSN: http://id.crossref.org/issn/13476947
• NDL BIB ID: 7899925
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7899925
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.70.980
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed