Biosynthesis of 2′-<i>O</i>-Methylmyxalamide D in the Myxobacterium<i>Cystobacter fuscus</i>: a Polyketide Synthase-Nonribosomal Peptide Synthetase System for the Myxalamide D Skeleton and a Methyltransferase for the Final<i>O</i>-Methylation

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  • FENG Zhiyang
    Graduate School of Bioagricultural Sciences, Nagoya University
  • QI Jianhua
    Graduate School of Bioagricultural Sciences, Nagoya University
  • TSUGE Takashi
    Graduate School of Bioagricultural Sciences, Nagoya University
  • OBA Yuichi
    Graduate School of Bioagricultural Sciences, Nagoya University
  • SAKAGAMI Youji
    Graduate School of Bioagricultural Sciences, Nagoya University
  • OJIKA Makoto
    Graduate School of Bioagricultural Sciences, Nagoya University

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  • Biosynthesis of 2'-O-Methylmyxalamide D in the Myxobacterium Cystobacter fuscus: a Polyketide Synthase-Nonribosomal Peptide Synthetase System for the Myxalamide D Skeleton and a Methyltransferase for the Final O-Methylation

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The biosynthetic gene cluster for the polyene antifungal antibiotic, 2′-O-methylmyxalamide D, was cloned from myxobacterium Cystobacter fuscus AJ-13278. A sequence analysis of the 12.8-kb region in the gene cluster revealed the presence of two type I polyketide synthase genes, mmxB and mmxC. The involvement of these two genes in the biosynthesis of 2′-O-methylmyxalamide D was confirmed by a gene disruption experiments. In addition, an S-adenosylmethionine-dependent methyltransferase gene (mmxM) was found downstream of the gene cluster and demonstrated, by a gene disruption analysis, to be responsible for converting the known unmethylated precursor, myxalamide D, into 2′-O-methylmyxalamide D.

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