Yeast Two-Hybrid Detection Systems That Are Highly Sensitive to a Certain Kind of Endocrine Disruptors
-
- Lee Haeng-Seog LEE Haeng-Seog
- Department of Biotechnology, University of Tokyo
-
- Sasagawa Shin-ichi SASAGAWA Shin-ichi
- Institute of Molecular and Cellular Biosciences, University of Tokyo
-
- Kato Shigeaki [他] KATO Shigeaki
- Institute of Molecular and Cellular Biosciences, University of Tokyo
-
- FUKUDA Ryouichi
- Department of Biotechnology, University of Tokyo
-
- HORIUCHI Hiroyuki
- Department of Biotechnology, University of Tokyo
-
- OHTA Akinori
- Department of Biotechnology, University of Tokyo
Access this Article
Search this Article
Author(s)
-
- Lee Haeng-Seog LEE Haeng-Seog
- Department of Biotechnology, University of Tokyo
-
- Sasagawa Shin-ichi SASAGAWA Shin-ichi
- Institute of Molecular and Cellular Biosciences, University of Tokyo
-
- Kato Shigeaki [他] KATO Shigeaki
- Institute of Molecular and Cellular Biosciences, University of Tokyo
-
- FUKUDA Ryouichi
- Department of Biotechnology, University of Tokyo
-
- HORIUCHI Hiroyuki
- Department of Biotechnology, University of Tokyo
-
- OHTA Akinori
- Department of Biotechnology, University of Tokyo
Abstract
We tested the effects of several combinations of bait and fish components of the yeast two-hybrid detection system for estrogenic activity. A combination of the full-length human estrogen receptor α with the nuclear receptor-binding domain of co-activator steroid receptor co-activator-1 (SRC-1) or transcriptional intermediate factor-2 (TIF-2) was most effective for estrogen-dependent induction of the chromosome-integrated UAS<SUB>GAL</SUB>-<I>CYC1</I><SUP>p</SUP>-<I>lacZ</I> reporter construct among the two-hybrid systems so far tested.
Journal
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 70(2), 521-524, 2006-02-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry
References: 20
-
1
- Transcriptional activation by estrogen receptor (ERα) and steroid receptor coactivator (SRC1) involves distinct mechanisms in yeast and mammalian cells
-
SHEPPARD H. M.
J. Mol. Endocrinol. 30, 411-422, 2003
Cited by (1)
-
2
- Synergism between ERalpha transactivation function 1 (AF-1) and AF-2 mediated by steroid receptor coactivator protein-1 : requirement for the AF-1 alpha-helical core and for a direct interaction between the N- and C-terminal domains
-
METIVIER R.
Mol. Endocrinol. 15, 1953-1970, 2001
Cited by (1)
-
3
- Ligands specify coactivator nuclear receptor (NR) box affinity for estrogen receptor subtypes
-
BRAMLETT K. S.
Mol. Endocrinol. 15, 909-922, 2001
Cited by (1)
-
4
- Employment of the Human Estrogen Receptor β Ligand-Binding Domain and Co-Activator SRC1 Nuclear Receptor-Binding Domain for the Construction of a Yeast Two-Hybrid Detection System for Endocrine Disrupters
-
LEE Haeng-Seog , MIYAUCHI Keisuke , NAGATA Yuji , FUKUDA Ryouichi , SASAGAWA Shin-ichi , ENDOH Hideki , KATO Shigeaki , HORIUCHI Hiroyuki , TAKAGI Masamichi , OHTA Akinori
The Journal of Biochemistry 131(3), 399-405, 2002-03-01
J-STAGE Ichushi Web References (39) Cited by (2)
-
5
- A nuclear receptor screening method using a steroid receptor co-activator-1 fragment in a yeast two-hybrid system
-
SASAGAWA S.
Analyt. Biochem. 289, 295-297, 2000
Cited by (2)
-
6
- Use of the yeast one-hybrid system to screen for mutations in the ligand-binding domain of the estrogen receptor
-
BUSH S. M.
Steroids 61, 102-109, 1996
Cited by (1)
-
7
- Promoter specificity of the two transcriptional activation functions of the human oestrogen receptor in yeast
-
METZGER D.
Nucl. Acids Res. 20, 2813-2817, 1992
Cited by (1)
-
8
- Characterization of the amino-terminal transcriptional activation function of the human estrogen receptor in animal and yeast cells
-
METZGER D.
J. Biol. Chem. 270, 9535-9542, 1995
Cited by (1)
-
9
- Nuclear receptor coactivators
-
GLASS C. K.
Curr Opin Cell Biol 9, 222-232, 1997
DOI Cited by (7)
-
10
- Ligand-specific transactivation of gene expression by a derivative of the human glucocorticoid receptor expressed in yeast
-
WRIGHT A. P. H.
J. Biol. Chem. 265, 14763-14769, 1990
Cited by (1)
-
11
- Rat liver c-erb A a1 thyroid hormone receptor is a constitutive activator in yeast (Saccharomyces cerevisiae) : essential role of domains D, E and F in hormone-independent transcription
-
OHASHI H.
Biochem. Biophys. Res. Commun. 178, 1167-1175, 1991
Cited by (1)
-
12
- An androgen-inducible expression system for Saccharomyces cerevisiae
-
PURVIS I. J.
Gene 106, 35-42, 1991
Cited by (1)
-
13
- <no title>
-
NISHIKAWA J.
Toxicol. Appl. Phrmacol. 154, 76-83, 1999
DOI Cited by (86)
-
14
- Environmental estrogens induce transcriptionally active estrogen receptor dimers in yeast : activity potentiated by the co-activator RIP140
-
SHEELER C. Q.
Environ. Health Perspect. 108, 97-103, 2000
Cited by (1)
-
15
- Selective interaction of vitamin D receptor with transcriptional coactivators by a vitamin D analog.
-
TAKEYAMA K.
Mol Cell Biol 19, 1049-1055, 1999
Cited by (17)
-
16
- A signature motif in transcriptional co-activators mediates binding to nuclear receptors
-
HEERY M.
Nature 387, 733-736, 1997
Cited by (13)
-
17
- Molecular cloning and characterization of rat estrogen receptor cDNA
-
KOIKE S.
Nucleic Acids Res. 15, 2499-2513, 1987
Cited by (25)
-
18
- Role of estrogen receptor beta in estrogen action
-
PETTERSSON K
Annu. Rev. Physiol. 63, 165-192, 2001
DOI Cited by (4)
-
19
- Potentiation of human estrogen receptor alpha-mediated gene expression by steroid receptor coactivator-1 (SRC-1) in Saccharomyces cerevisiae
-
ELLISON A. R.
J Steroid Biochem Mol Biol. 86, 15-26, 2003
Cited by (2)
-
20
- Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay
-
NISHIHARA Tsutomu , NISHIKAWA Jun-ichi , KANAYAMA Tomohiko , DAKEYAMA Fumi , SAITO Koichi , IMAGAWA Masayoshi , TAKATORI Satoshi , KITAGAWA Yoko , HORI Shinjiro , UTSUIMI Hideo
Journal of Health Science 46(4), 282-298, 2000-08-01
Ichushi Web References (28) Cited by (65)