Yeast Two-Hybrid Detection Systems That Are Highly Sensitive to a Certain Kind of Endocrine Disruptors

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Author(s)

Abstract

We tested the effects of several combinations of bait and fish components of the yeast two-hybrid detection system for estrogenic activity. A combination of the full-length human estrogen receptor α with the nuclear receptor-binding domain of co-activator steroid receptor co-activator-1 (SRC-1) or transcriptional intermediate factor-2 (TIF-2) was most effective for estrogen-dependent induction of the chromosome-integrated UAS<SUB>GAL</SUB>-<I>CYC1</I><SUP>p</SUP>-<I>lacZ</I> reporter construct among the two-hybrid systems so far tested.

Journal

  • Bioscience, Biotechnology, and Biochemistry

    Bioscience, Biotechnology, and Biochemistry 70(2), 521-524, 2006-02-23

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

References:  20

Codes

  • NII Article ID (NAID)
    10018534642
  • NII NACSIS-CAT ID (NCID)
    AA10824164
  • Text Lang
    ENG
  • Article Type
    NOT
  • ISSN
    09168451
  • NDL Article ID
    7833643
  • NDL Source Classification
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL Call No.
    Z53-G223
  • Data Source
    CJP  NDL  J-STAGE 
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