Yeast Two-Hybrid Detection Systems That Are Highly Sensitive to a Certain Kind of Endocrine Disruptors

• LEE Haeng-Seog

  Department of Biotechnology, University of Tokyo

• SASAGAWA Shin-ichi

  Institute of Molecular and Cellular Biosciences, University of Tokyo

• KATO Shigeaki

  Institute of Molecular and Cellular Biosciences, University of Tokyo

• FUKUDA Ryouichi

  Department of Biotechnology, University of Tokyo

• HORIUCHI Hiroyuki

  Department of Biotechnology, University of Tokyo

• OHTA Akinori

  Department of Biotechnology, University of Tokyo

Abstract

We tested the effects of several combinations of bait and fish components of the yeast two-hybrid detection system for estrogenic activity. A combination of the full-length human estrogen receptor α with the nuclear receptor-binding domain of co-activator steroid receptor co-activator-1 (SRC-1) or transcriptional intermediate factor-2 (TIF-2) was most effective for estrogen-dependent induction of the chromosome-integrated UAS_GAL-CYC1^p-lacZ reporter construct among the two-hybrid systems so far tested.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 70 (2), 521-524, 2006

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• endocrine disruptor
• estrogen
• yeast two-hybrid system
• human estrogen receptor-α (hERα)
• steroid receptor co-activator-1 (SRC-1)

Details

• CRID: 1390282681452494080
• NII Article ID: 10018534642
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.70.521
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 7833643
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7833643
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.70.521
• Web Site: https://search.jamas.or.jp/link/ui/2007048544
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed