Hydroxylation of Testosterone by Bacterial Cytochromes P450 Using the Escherichia coli Expression System

• AGEMATU Hitosi

  Bioresource Laboratories, Mercian Corp.

• MATSUMOTO Naoki

  Bioresource Laboratories, Mercian Corp.

• FUJII Yoshikazu

  Bioresource Laboratories, Mercian Corp.

• KABUMOTO Hiroki

  Bioresource Laboratories, Mercian Corp.

• DOI Satoru

  Bioresource Laboratories, Mercian Corp.

• MACHIDA Kazuhiro

  Bioresource Laboratories, Mercian Corp.

• ISHIKAWA Jun

  National Institute of Infectious Diseases

• ARISAWA Akira

  Bioresource Laboratories, Mercian Corp.

Bibliographic Information

Other Title

• Hydroxylation of Testosterone by Bacterial Cytochromes P450 Using the<i>Escherichia coli</i>Expression System
• Hydroxylation of testosterone by bacterial cytochrome P450 using the Escherichia coli expression system

Abstract

Two hundred thirteen cytochrome P450 (P450) genes were collected from bacteria and expressed based on an Escherichia coli expression system to test their hydroxylation ability to testosterone. Twenty-four P450s stereoselectively monohydroxylated testosterone at the 2α-, 2β-, 6β-, 7β-, 11β-, 12β-, 15β-, 16α-, and 17-positions (17-hydroxylation yields 17-ketoproduct). The hydroxylation site usage of the P450s is not the same as that of human P450s, while the 2α-, 2β-, 6β-, 11β-, 15β-, 16α-, and 17-hydroxylation are reactions common to both human and bacterial P450s. Most of the testosterone hydroxylation catalyzed by bacterial P450s is on the β face.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 70 (1), 307-311, 2006

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• cytochrome P450
• testosterone
• hydroxylation
• bioconversion

Details

• CRID: 1390282681452249984
• NII Article ID: 10018535933
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.70.307
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 7792141
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7792141
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.70.307
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed