Cyclic Mechanical Strain Induces Interleukin-6 Expression via Prostaglandin E2 Production by Cyclooxygenase-2 in MC3T3-E1 Osteoblast-like Cells
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- Narutomi Masanori
- Department of Oral Growth and Development, Division of Clinical Dentistry, Fukuoka Dental College
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- Nishiura Toshihiro
- Department of Functional Bioscience, Fukuoka Dental College
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- Sakai Toshio
- Department of Oral Growth and Development, Division of Clinical Dentistry, Fukuoka Dental College
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- Abe Kimio
- Department of Functional Bioscience, Fukuoka Dental College
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- Ishikawa Hiroyuki
- Department of Oral Growth and Development, Division of Clinical Dentistry, Fukuoka Dental College
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Interleukin (IL)-6, produced by osteoblasts, is one of the key cytokines that promote bone resorption. This study examined the effect of cyclic mechanical strain on the expression of IL-6 mRNA and protein, and the induction mechanism of IL-6 in MC3T3-E1 osteoblast-like cells using RT-PCR and ELISA. MC3T3-E1 cells were cultured in α-MEM with 10% FBS on flexible, collagen I-coated membranes and subjected to cyclic mechanical strain at 6 cycles/min. The production of IL-6 protein was increased at 3 h, reached a peak at 6 h, and was maintained up to 24 h by the cyclic mechanical strain. The strain increased the production of IL-6 protein in a force-dependent manner. The expression of IL-6 mRNA was increased by the cyclic mechanical strain. Prostaglandin (PG)E2 production was induced at 1 h, dramatically increased to 3 h, and was gradually increased from 3 to 24 h by the cyclic mechanical strain. The strain increased PGE2 production in a force-dependent manner. Treatment with PGE2 increased the production of IL-6 protein from MC3T3-E1 cells. The cyclic mechanical strain promoted cyclooxygenase (COX)-2 but not COX-1 mRNA expression. Pretreatment with indomethacin and NS-398 prevented PGE2 production and partly inhibited the production of IL-6 protein induced by the cyclic mechanical strain. These findings suggest that cyclic mechanical strain increases IL-6 expression in osteoblasts, the induction of which is in part mediated via PGE2 production by COX-2.
収録刊行物
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- Journal of Oral Biosciences
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Journal of Oral Biosciences 49 (1), 65-73, 2007
歯科基礎医学会
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詳細情報 詳細情報について
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- CRID
- 1390282680190311040
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- NII論文ID
- 130004475639
- 10018562995
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- NII書誌ID
- AA11896386
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- ISSN
- 18803865
- 13490079
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- 本文言語コード
- en
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- データソース種別
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- JaLC
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- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可