Enhanced susceptibility to Fas-mediated apoptosis by interferon-.GAMMA. in an oral squamous cell carcinoma cell line

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  • TAKEMI Toshiaki
    Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University
  • TAKIZAWA Kunio
    Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University
  • IWASE Masayasu
    Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University
  • NAGUMO Masao
    Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University

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  • インターフェロン‐γによる口腔へん平上皮癌細胞株のＦａｓ誘導アポトーシスへの感受性増強

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Abstract

Fas is widely expressed on the cell surface of many normal and neoplastic cells and induces apoptotic cell death in the presence of Fas ligand (FasL) or Fas-agonistic antibody CH11 (Fas-mediated apoptosis) Cancer cells constitutively expressing Fas are generally resistant to Fas-mediatedapoptosis. Recently, it has been reported that interferon-γ(IFN-γ) enhances Fas-mediated apoptosis. An oral squamous cell carcinoma cell line, NA, also constitutively expresses Fas on the plasma membrane and shows low susceptibility to Fas-agonistic antibody-induced apoptosis. This study was conducted to examine the effects of IFN-γ on Fas-mediated apoptosis, the expression of Fas and FasL, and the production of soluble Fas (sFas) in NA cells. The results revealed that a Fas-agonistic antibody, CH11, induced apoptosis of NA cells and IFN-γ enhanced susceptibility of NA cells to CH11-induced apoptosis. Further more, IFN-γ up-regulated Fas expression on NA cells without affecting the expression of FasL. A slight decrease in sFas expression was induced by treatment with IFN-γ. These results suggest that IFN-γ may enhance the susceptibility of NA cells to Fas-mediated apoptosis through the up-regulation of Fas.

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