Generation of superoxide by stimulation of human neutrophils with visible light

• Shizuma Toru

  Department of Physiology, School of Medicine, Tokai University

• Fukuyama Naoto

  Department of Physiology, School of Medicine, Tokai University

• Kotani Susumu

  Department of Physiology, School of Medicine, Tokai University

• Jujoh Shio

  Department of Physiology, School of Medicine, Tokai University

• Kimura Koji

  Department of Physiology, School of Medicine, Tokai University

• Myoujin Kazunori

  Department of Physiology, School of Medicine, Tokai University

• Hida Miho

  Tokai University Junior College of Nursing and Medical Technology

• Takebayashi Yoshihiro

  Eijin Clinic

• Kurata Yasuhisa

  Kurata Hospital

• Nakazawa Hiroe

  Department of Physiology, School of Medicine, Tokai University

• Mori Hidezo

  Department of Cardiac Physiology, National Cardiovascular Center

Bibliographic Information

Other Title

• 可視光線の好中球刺激によるスーパーオキシド生成

Abstract

Objective: Patients who undergo hemodialysis with shielding from light reportedly show lower oxidative stress. To elucidate the mechanism of this effect, we examined the generation of reactive oxygen species during the irradiation of human neutrophils with visible light.<br>Methods: A suspension of human neutrophils was irradiated for 60 minutes with visible light from a fluorescent lamp (light intensity at the suspension surface: 14, 000lux). Formation of superoxide in the irradiated suspension and a light-shielded control suspension was estimated based on a change in the absorbance of cytochrome C.<br>Results: The rate of superoxide formation in the irradiated suspension was 2.02nmol⋅10⁶cells/mL/min, while that in the light-shielded control suspension was 0.79nmol⋅10⁶cells/mL/min.<br>Conclusion: Shielding from light during hemodialysis is likely to reduce the generation of superoxide by visible light stimulation of neutrophils in the circulating blood.

Journal

• Nihon Toseki Igakkai Zasshi

  Nihon Toseki Igakkai Zasshi 38 (12), 1771-1773, 2005

  The Japanese Society for Dialysis Therapy

Keywords

• 好中球
• 遮光透析
• 可視光線
• 酸化ストレス
• スーパーオキシド

Details

• CRID: 1390282679653766400
• NII Article ID: 130003721875
• NII Article ID: 10018623083
• NII Book ID: AN10432053
• DOI: 10.4009/jsdt.38.1771
• ISSN: 1883082X
• ISSN: 13403451
• Web Site: https://search.jamas.or.jp/link/ui/2006126966
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed