N-Terminus Methylation of <i>Desulfovibrio vulgaris</i> (Miyazaki) F Cytochrome <i>c</i>3

• Shin Iida

  Department of Bioengineering, Tokyo Institute of Technology

• Yusuke Kawano

  Department of Bioengineering, Tokyo Institute of Technology

• Noriyuki Asakura

  Department of Bioengineering, Tokyo Institute of Technology

• Kenji Tabata

  Department of Bioengineering, Tokyo Institute of Technology

• Ichiro Okura

  Department of Bioengineering, Tokyo Institute of Technology

• Toshiaki Kamachi

  Department of Bioengineering, Tokyo Institute of Technology

抄録

Cytochrome c3 from Desulfovibrio vulgaris (Miyazaki) F is an electron-transfer protein between hydrogenase and ferredoxin and other types of cytochromes. In this study, an N-terminus of the cytochrome c3 has been identified as an N-methylated alanine residue by cleavage with peptidase, liquid chromatography/mass spectrometry (LC/MS) and nuclear magnetic resonance (NMR) analysis. Heterologous expression of the cytochrome c3 with Shewanella oneidensis TSP-C is not methylated, this indicate that the N-methylation occur in vivo in D. vulgaris.

収録刊行物

• Chemistry Letters

  Chemistry Letters 36 (1), 184-185, 2006-12-20

  Oxford University Press (OUP)

キーワード

• General Chemistry

詳細情報

• CRID: 1360002219110967808
• NII論文ID: 10018664051
• NII書誌ID: AA00603318
• DOI: 10.1246/cl.2007.184
• ISSN: 13480715
• ISSN: 03667022
• Web Site: https://academic.oup.com/chemlett/article-pdf/36/1/184/55593607/cl.2007.184.pdf
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