Efficiency of photosynthetic pigment analysis by HPLC with a mixture of 13 pigment standards

• Chikuchi Yuki

  Institute for Environmental Sciences JGC Plantech Aomori Co Ltd.

• Ueda Shinji

  Institute for Environmental Sciences

• Kondo Kunio

  Institute for Environmental Sciences

• Senga Yasuhiro

  School of MarineScience and Technology, Tokai University

Bibliographic Information

Other Title

• 13種類の植物プランクトン色素混合標準を用いたHPLC定量法の有用性
• 13シュルイ ノ ショクブツ プランクトン シキソ コンゴウ ヒョウジュン オ モチイタ HPLC テイリョウホウ ノ ユウヨウセイ

Abstract

In this study, a mixture of 13 pigment standards was quantified simultaneously using HPLC (High performance liquid chromatography) A standard of mixed 13 pigments (chlorophyll c3, chlorophyll c2, peridinin, 19'-butanoyloxyfucoxanthin, fucoxanthin, 19'-hexanoyloxyfucoxanthin, prasinoxanthin, diadinoxantliin, alloxantliin, diatoxanthin, zeaxanthin, chlorophyll b, chlorophyll a and β-carotene) was confirmed that peak of these pigments clearly were separated during twenty minutes. Acetone was better than ethanol as a dilution solvent of mixture pigment standards. Pigment analysis in field samples (freshwater lake and brackish water lake) was agreed with the results of phytoplankton composition identified by the microscope. It is judged that the identification of phytoplankton in field sample could be determined rapidly by this calibration standard.

Journal

• Journal of Advanced Marine Science and Technology Society

  Journal of Advanced Marine Science and Technology Society 12 (1), 27-35, 2006

  Advanced Marine Science and Technology Society

Keywords

• HPLC
• phytoplankton
• Photosynthetic pigment

Details

• CRID: 1390282763099914752
• NII Article ID: 10018709615
• NII Book ID: AA11607434
• DOI: 10.14928/amstec.12.1_27
• ISSN: 21883262
• ISSN: 13412752
• NDL BIB ID: 8879307
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8879307
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed