MEASUREMENT OF PLATELET CONCENTRATES BY A BACTERIAL DETECTION SYSTEM, BDS AND EBDS

DOI 1 Citations 11 References
  • Hiruma Kiyoshi
    Division of Transfusion and Cell Therapy, Tokyo Metropolitan Komagome Hospital
  • Ishii Kayo
    Division of Transfusion and Cell Therapy, Tokyo Metropolitan Komagome Hospital
  • Takahashi Naomi
    Division of Transfusion and Cell Therapy, Tokyo Metropolitan Komagome Hospital
  • Abe Kumiko
    Division of Transfusion and Cell Therapy, Tokyo Metropolitan Komagome Hospital
  • Takagi Tomoko
    Division of Transfusion and Cell Therapy, Tokyo Metropolitan Komagome Hospital
  • Takeda Toshio
    Division of Transfusion and Cell Therapy, Tokyo Metropolitan Komagome Hospital
  • Kunitomo Yukiko
    Division of Transfusion and Cell Therapy, Tokyo Metropolitan Komagome Hospital
  • Nakagawa Yoshiko
    Division of Transfusion and Cell Therapy, Tokyo Metropolitan Komagome Hospital
  • Ozawa Naohiro
    Division of Transfusion and Cell Therapy, Tokyo Metropolitan Komagome Hospital
  • Okuyama Yoshiki
    Division of Transfusion and Cell Therapy, Tokyo Metropolitan Komagome Hospital
  • Eduki Shoji
    Kawasumi Laboratories Incorporation
  • Oshige Takaaki
    Kawasumi Laboratories Incorporation

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Other Title
  • 血液製剤細菌検出装置ＢＤＳとｅＢＤＳによる血小板濃厚液の細菌汚染の検出

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Abstract

Prevention of bacterial contamination of blood products is an important issue in maintaining the safety of transfusion. In this study, we evaluated the performance of a bacterial detection system (BDS, Pall) and its successor system, the enhanced bacterial detection system (eBDS, Pall) by measuring 200 platelet concentrations (PCs) provided by the Japan Red Cross Blood Center. These systems measure oxygen concentrations consumed by bacteria, and can detect bacterial contamination.<br>One of 100PCs measured by BDS, and none of a second series of 100PCs measured by eBDS were positive for bacterial contamination. When a part of the positive product was measured by a bacterial culture system, BacT/ALERT (bioMerieux), no bacteria was observed after culture for seven days. We therefore judged this to be a false positive result and determined no bacterial contamination occurred in any of the 200PCs in this study. With the BDS and eBDS, culture samples could be started within 2 to 3 minutes of sampling from the PC.<br>It is considered that the eBDS is a useful screening test for bacterial contamination of blood products, because a high number of many samples can be handled promptly and the results are available within 24 hours of sampling.

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