Abundance and diversity of sulphate-reducing bacterioplankton in Lake Suigetsu, a meromictic lake in Fukui, Japan

• Kondo Ryuji

  Department of Marine Bioscience, Fukui Prefectural University

• Osawa Kyoko

  Department of Marine Bioscience, Fukui Prefectural University

• Mochizuki Lisa

  Department of Marine Bioscience, Fukui Prefectural University

• Fujioka Yukiyasu

  Department of Marine Bioscience, Fukui Prefectural University

• Butani Junki

  Department of Marine Bioscience, Fukui Prefectural University

Bibliographic Information

Other Title

• Abundance and diversity of sulphate-reducing bacterioplankton in Lake Suigetsu, a meromictic lake in Fukui

Abstract

The depth distribution of sulphate-reducing bacteria (SRB) in the water column of a meromictic lake, Lake Suigetsu, Fukui, Japan was investigated using quantitative competitive PCR targeting the gene coding for portions of the α-subunit of dissimilatory sulphite reductase (dsrA). The total bacterial cell density (DAPI count) was 5−13×10⁶ cells mL⁻¹ in the water column of the lake with maximum abundance occurring at the oxic-anoxic interface layer. SRB were not detected in oxic surface water using competitive PCR. SRB were found in the anoxic waters below the oxycline ranging from 10⁴ to 10⁵ cells mL⁻¹, accounting for 0.3–8.9% of the total bacteria. The SRB cell densities were higher than previously estimated using the most-probable-number (MPN) method. Sequencing of the cloned PCR product of dsrA showed the existence of different SRB groups in the anoxic water. The majority of the dsrA sequences were associated with the Desulfosarcina-Desulfococcus-Desulfonema group and members of the Desulfobulbaceae family. Other dsrA clones belonged to the Desulfomicrobium and Desulfovibrio species as well as to a deeply branched group in the dsrA tree with no representatives from previously isolated SRB groups. These SRB species appear to be important for the sulphur and carbon cycle in the anoxic waters of Lake Suigetsu.

Journal

• Plankton and Benthos Research

  Plankton and Benthos Research 1 (4), 165-177, 2006

  The Plankton Society of Japan, The Japanese Association of Benthology

Keywords

• competitive PCR
• dissimilatory sulphite reductase gene
• meromictic lake
• sulphate-reducing prokaryotes

Details

• CRID: 1390001205265329920
• NII Article ID: 10018808463
• NII Book ID: AA12130745
• DOI: 10.3800/pbr.1.165
• ISSN: 1882627X
• ISSN: 18808247
• ISSN: http://id.crossref.org/issn/18808247
• NDL BIB ID: 8567657
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8567657
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed